Martin Ashley
Cancer Research UK Institute for Cancer Studies, University of Birmingham, UK.
Methods Mol Biol. 2005;298:227-40. doi: 10.1385/1-59259-877-3:227.
The focus of this chapter is the isolation and analysis of subsets of proteins or subproteomes. These methodologies can be applied to address fundamental questions in many areas of biological research. Protocols are provided for common techniques that include protein isolation from mammalian cells and the subsequent identification of peptides derived from endogenous proteins by the action of proteases.
本章的重点是蛋白质亚群或亚蛋白质组的分离与分析。这些方法可用于解决生物学研究许多领域中的基本问题。文中提供了一些常用技术的实验方案，包括从哺乳动物细胞中分离蛋白质，以及随后通过蛋白酶的作用鉴定源自内源性蛋白质的肽段。
