Thevis Mario, Loo Joseph A, Loo Rachel R Ogorzalek, Schänzer Wilhelm
Institute of Biochemistry-Center for Preventive Doping Research, German Sport University Cologne, Carl-Diem Weg 6, 50933 Cologne, Germany.
Rapid Commun Mass Spectrom. 2007;21(3):297-304. doi: 10.1002/rcm.2848.
Mass spectrometry has become an invaluable tool for the identification of prohibited peptide hormones and proteins in doping control analysis. Regulatory authorities have established criteria for identifying banned drugs in doping control specimens, but these criteria do not address the specific issues for high molecular weight protein drugs such as molecular weight determination of multiply charged molecules, analysis of chemically or enzymatically derived degradation products, identification of amino acid sequence tags, etc. Technical considerations such as sample preparation methods (e.g. immunoaffinity purification), resulting analytes (e.g. intact compounds vs. chemically or enzymatically derived peptides), ionization modes, analyzer resolution, and the information provided by respective techniques are discussed in light of sports drug testing requirements using typical application examples.
质谱分析法已成为兴奋剂检测分析中鉴定违禁肽类激素和蛋白质的一项极为重要的工具。监管机构已制定了在兴奋剂检测样本中识别违禁药物的标准，但这些标准并未涉及高分子量蛋白质药物的具体问题，如多电荷分子的分子量测定、化学或酶促衍生降解产物的分析、氨基酸序列标签的识别等。本文结合体育药物检测要求，通过典型应用实例，讨论了诸如样品制备方法（如免疫亲和纯化）、所得分析物（如完整化合物与化学或酶促衍生肽）、电离模式、分析仪分辨率以及各技术所提供信息等技术考量因素。
