Lane R J, Maskrey P, Nicholson G A, Siddiqui P Q, Nicholson M, Gascoigne P, Pennington R J, Gardner-Medwin D, Walton J N
J Neurol Sci. 1979 Nov;43(3):377-94. doi: 10.1016/0022-510x(79)90017-0.
Some recently described abnormalities in the serum and red cell membranes in Duchenne dystrophy have been examined as methods of carrier detection in a single-blind controlled study. Twelve carriers (4 definite, 3 probable and 5 possible carriers previously found to have raised creatine kinase levels) and 12 normal female controls of the same age, were examined on 3 separate occasions at approximately two-weekly intervals. Analysis of age-dependent red cell shape changes, serum haemopexin levels, red cell K+ efflux rate, sensitivity of red cell ghost membrane ATPase to ouabain, membrane protein phosphorylation studies and lactate dehydrogenase isoenzyme profiles on agarose gel electrohoresis all failed to distinquish carriers from controls. The carriers suffered muscle cramps more frequently than the controls and all but one carrier and two control subjects were correctly identified by manual muscle strength testing, certain proximal muscles in paricular being consistently weaker in carriers than in the control group subjects. Scalar electrocardiography revealed higher values for the R/S ratio in Leads V1 and V2 and the sum (R-S) in V2.
在一项单盲对照研究中，对一些最近所描述的杜氏肌营养不良患者血清和红细胞膜异常情况进行了检测，作为携带者检测方法。选取了12名携带者（4名确定携带者、3名可能携带者和5名此前发现肌酸激酶水平升高的可能携带者）以及12名年龄相同的正常女性对照者，在大约两周的间隔时间内分3次进行检查。对年龄依赖性红细胞形态变化、血清血红素结合蛋白水平、红细胞钾离子外流率、红细胞空影膜ATP酶对哇巴因的敏感性、膜蛋白磷酸化研究以及琼脂糖凝胶电泳上的乳酸脱氢酶同工酶谱进行分析，均未能将携带者与对照者区分开来。携带者比对照者更频繁地出现肌肉痉挛，除一名携带者和两名对照者外，通过徒手肌力测试均可正确识别所有携带者，特别是某些近端肌肉，携带者始终比对照组受试者更弱。标测心电图显示，V1和V2导联的R/S比值以及V2导联的（R - S）总和值更高。
