Czarnecka Beata, Nicholson John W
Department of Biomaterials and Experimental Dentistry, University of Medical Sciences, Poznan, Poland.
Dent Mater. 2006 Feb;22(2):133-7. doi: 10.1016/j.dental.2005.04.008. Epub 2005 Jul 19.
The aim of the study was to determine how the presence of low concentrations of the amino acids glycine and alanine affected the interaction of restorative glass-ionomer cements with water.
For each material (Fuji IX and Ketac Molar, both handmixed), sets of six cylindrical specimens of 4 mm diameter x 6 mm height were prepared and weighed; then stored individually in one of: deionized water, 0.1% by mass glycine, 0.5% glycine, 0.1% alanine (Sigma Aldrich) and 0.5% alanine. After 1 week, the specimens were re-weighed and the pH of each solution determined. The solutions were then bulked and analyzed for ion content (Na, Ca, Al, Si and P) using ICP-OES. In a separate series of experiments, disks (12 mm diameter x 1 mm thick) were prepared, and exposed to similar solutions for periods of a week, after which Vicker's hardness was determined. Differences were analysed by one-way ANOVA, followed by Neumann-Keuls post-hoc analysis (P<0.05).
All specimens increased in weight, and altered pH of the storage solution to similar values. Both cements released less aluminum, but generally more sodium, calcium and silicon in the presence of amino acids. Little or no phosphorus was found to be released under any conditions. Surface hardness of Fuji IX was not influenced by the presence of amino acids, but for Ketac Molar it was generally lower in the presence of amino acids.
These results show that the interaction of glass-ionomer cements with water is influenced by the presence of low concentrations of amino acids, and that this may alter surface hardness.
本研究旨在确定低浓度氨基酸甘氨酸和丙氨酸的存在如何影响修复性玻璃离子水门汀与水的相互作用。
对于每种材料（均为手工混合的Fuji IX和Ketac Molar），制备六组直径4 mm×高6 mm的圆柱形试件并称重；然后将其分别储存在以下溶液之一中：去离子水、质量分数0.1%的甘氨酸、0.5%的甘氨酸、0.1%的丙氨酸（西格玛奥德里奇公司）和0.5%的丙氨酸。1周后，再次称量试件重量并测定每种溶液的pH值。然后将溶液合并，使用电感耦合等离子体发射光谱法（ICP - OES）分析离子含量（钠、钙、铝、硅和磷）。在另一系列实验中，制备圆盘（直径12 mm×厚1 mm），并将其暴露于类似溶液中一周，之后测定维氏硬度。采用单向方差分析进行差异分析，随后进行纽曼 - 基尔斯事后分析（P<0.05）。
所有试件重量增加，且储存溶液的pH值改变为相似值。在氨基酸存在的情况下，两种水门汀释放的铝较少，但通常释放更多的钠、钙和硅。在任何条件下均未发现有磷释放或释放量极少。Fuji IX的表面硬度不受氨基酸存在的影响，但对于Ketac Molar，在氨基酸存在时其表面硬度通常较低。
这些结果表明，低浓度氨基酸的存在会影响玻璃离子水门汀与水的相互作用，且这可能会改变表面硬度。
