Theamboonlers A, Veravigrom M, Yambangyang O, Trairatvorakul P, Chongsrisawat V, Poovorawan Y
Viral Hepatitis Research Unit, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Acta Virol. 2005;49(2):111-5.
Rotaviruses are the leading cause of severe gastroenteritis among infants and young children worldwide. Between November 2002 and March 2004, 36 stool specimens of 108 children with acute diarrhea in Bangkok, Thailand were found positive for Rotavirus A (RV-A) by RT-PCR. The 36 isolates were subjected to genotyping by RFLP analysis and direct sequencing of a part of the gene for major outer capsid glycoprotein VP7. The sequences obtained were subjected to phylogenetic analysis. Among the isolates the genotypes G1 (5.6%), G2 (69.4 %) and G9 (25.0 %) were found. Comparison of these results with those of previous studies covering the period of 1982-1999 revealed a changing pattern of RV-A G genotypes and thus contributed to the understanding of RV-A epidemiology in Thailand. Any vaccine to be developed against this virus should target the G9 genotype as one of common global genotypes.
轮状病毒是全球婴幼儿严重肠胃炎的主要病因。2002年11月至2004年3月期间，泰国曼谷108名急性腹泻儿童的36份粪便样本经逆转录聚合酶链反应（RT-PCR）检测，发现A组轮状病毒（RV-A）呈阳性。对这36株分离株进行限制性片段长度多态性（RFLP）分析及主要外衣壳糖蛋白VP7部分基因的直接测序进行基因分型，并对所得序列进行系统发育分析。在这些分离株中，发现了G1（5.6%）、G2（69.4%）和G9（25.0%）基因型。将这些结果与1982 - 1999年期间以前的研究结果相比较，揭示了RV-A G基因型的变化模式，从而有助于了解泰国的RV-A流行病学情况。任何针对这种病毒研发的疫苗都应将G9基因型作为常见的全球基因型之一作为靶点。
