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大肠杆菌K12部分二倍体中rac基因座DNA的缺失。

Loss of rac locus DNA in merozygotes of Escherichia coli K12.

作者信息

Evans R, Seeley N R, Kuempel P L

出版信息

Mol Gen Genet. 1979 Oct 1;175(3):245-50. doi: 10.1007/BF00397223.

Abstract

DNA-DNA hybridization was used to demonstrate that the substituted DNA in the bacteriophage lambda recE (formerly called lambda reverse) is homologous to DNA at the rac locus in Escherichia coli. Strains that are rac- do not contain appreciable amounts of this DNA, and it is lost from a rac+ episome (F' 123) after transmission to a rac- recipient. This is consistent with the proposal that the rac locus contains a cryptic prophage (Low, 1973).

摘要

采用DNA-DNA杂交技术来证明噬菌体λrecE(以前称为λ反向)中的取代DNA与大肠杆菌rac位点的DNA具有同源性。rac-菌株不含大量此类DNA,且在传递给rac-受体后,它会从rac+附加体(F' 123)中丢失。这与rac位点含有一个隐蔽原噬菌体的提议一致(洛,1973年)。

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