Nakashima Yasuhiro, Tagawa Hiroyuki, Suzuki Ritsuro, Karnan Sivasundaram, Karube Kennosuke, Ohshima Koichi, Muta Koichiro, Nawata Hajime, Morishima Yasuo, Nakamura Shigeo, Seto Masao
Division of Molecular Medicine, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Aichi, Japan.
Genes Chromosomes Cancer. 2005 Nov;44(3):247-55. doi: 10.1002/gcc.20245.
Natural killer (NK) cell lymphomas/leukemias are highly aggressive lymphoid malignancies, but little is known about their genomic alterations, and thus there is an urgent need for identification and analysis of NK cell lymphomas/leukemias. Recently, we developed our own array-based comparative genomic hybridization (array CGH) with an average resolution of 1.3 Mb. We performed an array CGH analysis for 27 NK-cell lymphoma/leukemia cases that were classified into two disease groups based on the World Health Organization Classification (10 aggressive NK-cell leukemia cases and 17 extranodal NK/T-cell [NK/T] lymphomas, nasal type). We identified the differences in the genomic alteration patterns of the two groups. The recurrent regions characteristic of the aggressive NK-cell leukemia group compared with those of the extranodal NK/T lymphoma, nasal-type group, were gain of 1q and loss of 7p15.1-p22.3 and 17p13.1. In particular, gain of 1q23.1-24.2 (P = 0.041) and 1q31.3-q44 (P = 0.003-0.047), and loss of 7p15.1-p22.3 (P = 0.012-0.041) and 17p13.1 (P = 0.012) occurred significantly more frequently in the former than in the latter group. Recurrent regions characteristic of the extranodal NK/T lymphoma, nasal-type group, compared with those of the other group were gain of 2q, and loss of 6q16.1-q27, 11q22.3-q23.3, 5p14.1-p14.3, 5q34-q35.3, 1p36.23-p36.33, 2p16.1-p16.3, 4q12, and 4q31.3-q32.1. Our results can be expected to provide further insights into the genetic basis of lymphomagenesis and the clinicopathologic features of NK-cell lymphomas/leukemias.
自然杀伤（NK）细胞淋巴瘤/白血病是侵袭性很强的淋巴系统恶性肿瘤，但人们对其基因组改变了解甚少，因此迫切需要对NK细胞淋巴瘤/白血病进行鉴定和分析。最近，我们开发了自己的基于芯片的比较基因组杂交技术（芯片比较基因组杂交，array CGH），平均分辨率为1.3兆碱基对。我们对27例NK细胞淋巴瘤/白血病病例进行了芯片比较基因组杂交分析，这些病例根据世界卫生组织分类法分为两个疾病组（10例侵袭性NK细胞白血病病例和17例结外NK/T细胞[NK/T]淋巴瘤，鼻型）。我们确定了两组基因组改变模式的差异。与结外NK/T淋巴瘤鼻型组相比，侵袭性NK细胞白血病组特有的反复出现的区域为1q获得以及7p15.1-p22.3和17p13.1缺失。特别是，1q23.1-24.2（P = 0.041）和1q31.3-q44（P = 0.003 - 0.047）的获得以及7p15.1-p22.3（P = 0.012 - 0.041）和17p13.1（P = 0.012）的缺失在前一组中出现的频率明显高于后一组。与另一组相比，结外NK/T淋巴瘤鼻型组特有的反复出现的区域为2q获得以及6q16.1-q27、11q22.3-q23.3、5p14.1-p14.3、5q34-q35.3、1p36.23-p36.33、2p16.1-p16.3、4q12和4q31.3-q32.1缺失。我们的结果有望为淋巴瘤发生的遗传基础以及NK细胞淋巴瘤/白血病的临床病理特征提供进一步的见解。
