Department of Internal Medicine, Veterinary Faculty, Harran University, Şanlıurfa, Turkey.
Department of Internal Medicine, Veterinary Faculty, Selçuk University, Konya, Turkey.
Vet Med Sci. 2023 Nov;9(6):2420-2429. doi: 10.1002/vms3.1299. Epub 2023 Oct 24.
To investigate intestinal injury, repair and vasculitis biomarkers that may illuminate the progression and/or pathogenesis of feline infectious peritonitis (FIP) or feline enteric coronavirus (FECV) infection.
A total of 40 cats with effusive FIP (30 with abdominal effusion, AE group; 10 with thoracic effusion, TE group) and 10 asymptomatic but FECV positive cats (FECV group), all were confirmed by reverse transcription polymerase chain reaction either in faeces or effusion samples. Physical examinations and effusion tests were performed. Trefoil factor-3 (TFF-3), intestinal alkaline phosphatase (IAP), intestinal fatty acid binding protein (I-FABP), myeloperoxidase-anti-neutrophilic cytoplasmic antibody (MPO-ANCA) and proteinase 3-ANCA (PR3-ANCA) concentrations were measured both in serum and effusion samples.
Rectal temperature and respiratory rate were highest in the TE group (p < 0.000). Effusion white blood cell count was higher in the AE group than TE group (p < 0.042). Serum TFF-3, IAP and I-FABP concentrations were higher in cats with effusive FIP than the cats with FECV (p < 0.05). Compared with the AE group, TE group had lower effusion MPO-ANCA (p < 0.036), higher IAP (p < 0.050) and higher TFF-3 (p < 0.016) concentrations.
Markers of intestinal and epithelial surface injury were higher in cats with effusive FIP than those with FECV. Compared to cats with abdominal effusions, markers of apoptosis inhibition and immunostimulation to the injured epithelium were more potent in cats with thoracic effusion, suggesting the possibility of a poorer prognosis or more advanced disease in these patients.
研究可能阐明猫传染性腹膜炎（FIP）或猫传染性肠炎冠状病毒（FECV）感染进展和/或发病机制的肠道损伤、修复和血管炎生物标志物。
总共 40 只患有渗出性 FIP 的猫（30 只患有腹水，AE 组；10 只患有胸腔积液，TE 组）和 10 只无症状但 FECV 阳性的猫（FECV 组），均通过粪便或渗出液样本的逆转录聚合酶链反应（RT-PCR）得到确认。进行了体格检查和渗出液检查。测量了血清和渗出液样本中三叶因子 3（TFF-3）、肠碱性磷酸酶（IAP）、肠脂肪酸结合蛋白（I-FABP）、髓过氧化物酶-抗中性粒细胞胞质抗体（MPO-ANCA）和蛋白酶 3-抗中性粒细胞胞质抗体（PR3-ANCA）的浓度。
TE 组的直肠温度和呼吸频率最高（p<0.000）。AE 组的渗出液白细胞计数高于 TE 组（p<0.042）。患有渗出性 FIP 的猫的血清 TFF-3、IAP 和 I-FABP 浓度高于患有 FECV 的猫（p<0.05）。与 AE 组相比，TE 组的胸腔积液 MPO-ANCA 较低（p<0.036），IAP 较高（p<0.050），TFF-3 较高（p<0.016）。
患有渗出性 FIP 的猫的肠道和上皮表面损伤标志物高于患有 FECV 的猫。与患有腹部积液的猫相比，患有胸腔积液的猫的凋亡抑制和对受损上皮的免疫刺激标志物更强，这表明这些患者的预后可能更差或疾病更严重。
