细胞迁移的体外和体内成像：两种相互依存的方法来揭示转移形成机制

In vitro and in vivo imaging of cell migration: two interdepending methods to unravel metastasis formation.

作者信息

Palm Daniel, Lang Kerstin, Brandt Burkhard, Zaenker Kurt S, Entschladen Frank

机构信息

Institute of Immunology, Witten/Herdecke University, Stockumer Str. 10, 58448 Witten, Germany.

出版信息

Semin Cancer Biol. 2005 Oct;15(5):396-404. doi: 10.1016/j.semcancer.2005.06.008.

DOI:10.1016/j.semcancer.2005.06.008

PMID:16054391

Abstract

Metastasis development requires the migratory activity of tumor cells. It is therefore important to understand the molecular mechanisms of this migration in order to prevent metastasis development, which is the pernicious step in most solid tumor diseases. A lot of methods have been invented to investigate tumor cell migration, but not all are equally suited and no method alone is able to deliver a complete picture of tumor cell migration. We herein suggest a combination of three-dimensional in vitro and in vivo methods for the investigation of tumor cell migration and summarize the knowledge, which has been reached so far.

摘要

转移的发生需要肿瘤细胞的迁移活性。因此，了解这种迁移的分子机制对于预防转移的发生至关重要，而转移是大多数实体瘤疾病中的有害步骤。已经发明了许多方法来研究肿瘤细胞迁移，但并非所有方法都同样适用，而且没有一种方法能够单独全面地呈现肿瘤细胞迁移的情况。我们在此建议结合三维体外和体内方法来研究肿瘤细胞迁移，并总结迄今为止所获得的知识。