Martinez-Pastor Felipe, Diaz-Corujo Ana R, Anel Enrique, Herraez Paz, Anel Luis, de Paz Paulino
Cell Biology and Anatomy, University of León, 24071 León, Spain.
Theriogenology. 2005 Sep 1;64(4):958-74. doi: 10.1016/j.theriogenology.2005.01.003.
We have studied the effect of post mortem time and season on sperm subpopulation pattern and characteristics. We used epididymal samples from free-ranging Iberian red deers harvested during the hunting season. We studied samples at different moments of the year (rut, transition period and post-rut), and at different times post mortem (up to 4 days). Sperm were extracted from the cauda epididymis and their motility was evaluated by means of a CASA system. A principal component and clustering analysis were carried out to identify subpopulations. Post mortem time caused a significant decrease in motility quality, and a general deterioration in subpopulation characteristics. We found three subpopulations the first day, and the one indicating good sperm quality decreased with post mortem time until it disappeared on the fourth day. This may indicate considerable impairment of the samples after 72 h post mortem, which could compromise their use in AI programs. With regard to season, subpopulation pattern and characteristics were better in the transition and post-rut periods. Moreover, we found one subpopulation formed by mature spermatozoa, which increased from rut to post-rut. This might be a negative fact, because samples collected after the rut may undergo hypermaturation, which possibly impairs fertility. Our results are of interest for the management of wildlife germplasm banks based on post mortem sperm recovery.
我们研究了死后时间和季节对精子亚群模式及特征的影响。我们使用了在狩猎季节捕获的自由放养的伊比利亚马鹿的附睾样本。我们研究了一年中不同时刻（发情期、过渡期和发情后期）以及死后不同时间（长达4天）的样本。从附睾尾部提取精子，并通过计算机辅助精子分析（CASA）系统评估其活力。进行主成分分析和聚类分析以识别亚群。死后时间导致活力质量显著下降，亚群特征普遍恶化。第一天我们发现了三个亚群，其中表示精子质量良好的亚群随着死后时间的延长而减少，直至在第四天消失。这可能表明死后72小时后样本受到了相当大的损害，这可能会影响它们在人工授精程序中的应用。关于季节，亚群模式和特征在过渡期和发情后期更好。此外，我们发现了一个由成熟精子组成的亚群，其数量从发情期到发情后期有所增加。这可能是一个负面因素，因为发情期后采集的样本可能会经历过度成熟，这可能会损害生育能力。我们的研究结果对于基于死后精子回收的野生动物种质库管理具有重要意义。
