• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过克隆和缺失技术在鼠伤寒沙门氏菌和大肠杆菌中定位RNA聚合酶σ亚基的基因。

Gene for the RNA polymerase sigma subunit mapped in Salmonella typhimurium and Escherichia coli by cloning and deletion.

作者信息

Scaife J G, Heilig J S, Rowen L, Calendar R

出版信息

Proc Natl Acad Sci U S A. 1979 Dec;76(12):6510-4. doi: 10.1073/pnas.76.12.6510.

DOI:10.1073/pnas.76.12.6510
PMID:160566
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC411895/
Abstract

The genes for the RNA polymerase sigma subunit (rpoD) and DNA primase (dnaG) of Salmonella typhimurium have been cloned into lambda vectors. Combined restriction, deletion and functional analysis of the cloned fragment allows us to map the genes precisely on the fragment, establishes the direction in which rpoD is transcribed, and reveals the existence of at least one new gene in the vicinity. A closely homologous, smaller fragment of Escherichia coli DNA, also cloned into lambda, contains rpoD and at least part of dnaG.

摘要

鼠伤寒沙门氏菌RNA聚合酶σ亚基（rpoD）和DNA引发酶（dnaG）的基因已被克隆到λ载体中。对克隆片段进行限制性内切酶联合分析、缺失分析和功能分析，使我们能够在该片段上精确绘制这些基因图谱，确定rpoD的转录方向，并揭示其附近至少存在一个新基因。同样克隆到λ载体中的一段与大肠杆菌DNA密切同源的较小片段，包含rpoD和至少部分dnaG。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af3b/411895/486af36da669/pnas00012-0497-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af3b/411895/7e369f7bbeac/pnas00012-0496-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af3b/411895/63b240378ace/pnas00012-0496-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af3b/411895/116ab342a535/pnas00012-0497-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af3b/411895/486af36da669/pnas00012-0497-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af3b/411895/7e369f7bbeac/pnas00012-0496-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af3b/411895/63b240378ace/pnas00012-0496-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af3b/411895/116ab342a535/pnas00012-0497-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af3b/411895/486af36da669/pnas00012-0497-b.jpg

相似文献

1
Gene for the RNA polymerase sigma subunit mapped in Salmonella typhimurium and Escherichia coli by cloning and deletion.通过克隆和缺失技术在鼠伤寒沙门氏菌和大肠杆菌中定位RNA聚合酶σ亚基的基因。
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6510-4. doi: 10.1073/pnas.76.12.6510.
2
Salmonella typhimurium rpoB and rpoC genes cloned on lambda phages.
FEBS Lett. 1985 Jun 3;185(1):33-6. doi: 10.1016/0014-5793(85)80735-3.
3
Nucleotide sequence of the rpsU-dnaG-rpoD operon from Salmonella typhimurium and a comparison of this sequence with the homologous operon of Escherichia coli.鼠伤寒沙门氏菌rpsU-dnaG-rpoD操纵子的核苷酸序列及其与大肠杆菌同源操纵子的序列比较。
Gene. 1985;40(1):67-78. doi: 10.1016/0378-1119(85)90025-3.
4
Regulation of expression of the Escherichia coli dnaG gene and amplification of the dnaG primase.大肠杆菌dnaG基因表达的调控及dnaG引发酶的扩增
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4907-11. doi: 10.1073/pnas.79.16.4907.
5
Possible new genes as revealed by molecular analysis of a 5-kb Escherichia coli chromosomal region 5' to the rpsU-dnaG-rpoD macromolecular-synthesis operon.通过对rpsU-dnaG-rpoD大分子合成操纵子上游5kb大肠杆菌染色体区域进行分子分析所揭示的潜在新基因。
Gene. 1987;51(2-3):149-61. doi: 10.1016/0378-1119(87)90303-9.
6
Cloning and characterization of the Escherichia coli chromosomal region surrounding the dnaG Gene, with a correlated physical and genetic map of dnaG generated via transposon Tn5 mutagenesis.大肠杆菌dnaG基因周围染色体区域的克隆与特性分析，以及通过转座子Tn5诱变产生的dnaG相关物理图谱和遗传图谱。
Mol Gen Genet. 1982;185(1):120-8. doi: 10.1007/BF00333800.
7
A gene from Escherichia coli affecting the sigma subunit of RNA polymerase.一种来自大肠杆菌的影响RNA聚合酶σ亚基的基因。
Proc Natl Acad Sci U S A. 1977 May;74(5):1836-40. doi: 10.1073/pnas.74.5.1836.
8
The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12.来自大肠杆菌K12的RNA聚合酶σ亚基的克隆rpoD基因的核苷酸序列。
Nucleic Acids Res. 1981 Jun 25;9(12):2889-903. doi: 10.1093/nar/9.12.2889.
9
Overexpression and purification of the sigma subunit of Escherichia coli RNA polymerase.大肠杆菌RNA聚合酶σ亚基的过表达与纯化
Gene. 1983 Dec;26(2-3):109-18. doi: 10.1016/0378-1119(83)90180-4.
10
Isolation and characterization of transducing phage coding for sigma subunit of Escherichia coli RNA polymerase.编码大肠杆菌RNA聚合酶σ亚基的转导噬菌体的分离与特性分析。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5789-93. doi: 10.1073/pnas.76.11.5789.

引用本文的文献

1
Amber mutations in the structural gene for RNA polymerase sigma factor of Escherichia coli.大肠杆菌RNA聚合酶σ因子结构基因中的琥珀突变。
Mol Gen Genet. 1980;180(2):293-300. doi: 10.1007/BF00425841.
2
Hybrid plasmid carrying Escherichia coli genes for the primase (dnaG) and RNA polymerase sigma factor (rpoD); gene organization and control of their expression.携带大肠杆菌引发酶（dnaG）和RNA聚合酶σ因子（rpoD）基因的杂交质粒；基因组织及其表达调控。
Mol Gen Genet. 1980;178(3):487-97. doi: 10.1007/BF00337853.
3
Amber dnaG mutation exerting a polar effect on the synthesis of RNA polymerase sigma factor in Escherichia coli.

本文引用的文献

1
ISOLATION OF THE lambda PHAGE REPRESSOR.λ噬菌体阻遏物的分离
Proc Natl Acad Sci U S A. 1967 Feb;57(2):306-13. doi: 10.1073/pnas.57.2.306.
2
Deletion mutants of bacteriophage lambda. 3. Physical structure of att-phi.噬菌体λ的缺失突变体。3. att-phi的物理结构。
J Mol Biol. 1971 Mar 14;56(2):403-23. doi: 10.1016/0022-2836(71)90473-6.
3
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
琥珀型dnaG突变对大肠杆菌RNA聚合酶σ因子的合成产生极性效应。
Mol Gen Genet. 1984;196(1):179-82. doi: 10.1007/BF00334114.
4
Intragenic localization of amber and temperature-sensitive rpoD mutations affecting RNA polymerase sigma factor of Escherichia coli.影响大肠杆菌RNA聚合酶σ因子的琥珀突变和温度敏感rpoD突变的基因内定位。
Mol Gen Genet. 1983;189(2):193-8. doi: 10.1007/BF00337803.
5
Linkage map of Salmonella typhimurium, Edition VI.鼠伤寒沙门氏菌连锁图谱，第六版。
Microbiol Rev. 1983 Sep;47(3):410-53. doi: 10.1128/mr.47.3.410-453.1983.
6
Isolation and physical mapping of the gene encoding the major sigma factor of Bacillus subtilis RNA polymerase.枯草芽孢杆菌RNA聚合酶主要σ因子编码基因的分离与物理图谱绘制。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4074-8. doi: 10.1073/pnas.80.13.4074.
7
Cloning of bacterial DNA replication genes in bacteriophage lambda.细菌DNA复制基因在噬菌体λ中的克隆
Mol Gen Genet. 1982;187(3):501-9. doi: 10.1007/BF00332635.
8
The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12.来自大肠杆菌K12的RNA聚合酶σ亚基的克隆rpoD基因的核苷酸序列。
Nucleic Acids Res. 1981 Jun 25;9(12):2889-903. doi: 10.1093/nar/9.12.2889.
9
Regulation of expression of the Escherichia coli dnaG gene and amplification of the dnaG primase.大肠杆菌dnaG基因表达的调控及dnaG引发酶的扩增
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4907-11. doi: 10.1073/pnas.79.16.4907.
10
Multiple procaryotic ribonucleic acid polymerase sigma factors.多种原核生物核糖核酸聚合酶σ因子
Microbiol Rev. 1986 Sep;50(3):227-43. doi: 10.1128/mr.50.3.227-243.1986.
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
4
The steric effect in lysogenization by bacteriophage lambda. I. Lysogenization of a partially diploid strain of Escherichia coli K-12.λ噬菌体溶原化过程中的空间效应。I. 大肠杆菌K-12部分二倍体菌株的溶原化
Virology. 1965 Nov;27(3):329-39. doi: 10.1016/0042-6822(65)90112-1.
5
Genetics of the Enterobacteriaceae. C. Molecular relationships among members of the Enterobacteriaceae.肠杆菌科的遗传学。C. 肠杆菌科成员间的分子关系。
Adv Genet. 1971;16:81-118. doi: 10.1016/s0065-2660(08)60355-7.
6
Escherichia coli mutants temperature-sensitive for DNA synthesis.对DNA合成温度敏感的大肠杆菌突变体。
Mol Gen Genet. 1971;113(3):273-84. doi: 10.1007/BF00339547.
7
Establishment and maintenance of repression by bacteriophage lambda: the role of the cI, cII, and c3 proteins.噬菌体λ介导的基因沉默的建立与维持：cI、cII和c3蛋白的作用
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2190-4. doi: 10.1073/pnas.68.9.2190.
8
Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants.核酸内切酶I缺陷型和核糖核酸酶I缺陷型大肠杆菌突变体。
J Mol Biol. 1968 Jul 14;34(2):331-46. doi: 10.1016/0022-2836(68)90257-x.
9
Pedigrees of some mutant strains of Escherichia coli K-12.大肠杆菌K-12某些突变菌株的谱系。
Bacteriol Rev. 1972 Dec;36(4):525-57. doi: 10.1128/br.36.4.525-557.1972.
10
Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization.噬菌体λ的缺失突变体。I. 分离与初步表征。
J Mol Biol. 1971 Mar 14;56(2):369-84. doi: 10.1016/0022-2836(71)90471-2.