来自大肠杆菌K12的RNA聚合酶σ亚基的克隆rpoD基因的核苷酸序列。
The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12.
作者信息
Burton Z, Burgess R R, Lin J, Moore D, Holder S, Gross C A
出版信息
Nucleic Acids Res. 1981 Jun 25;9(12):2889-903. doi: 10.1093/nar/9.12.2889.
Abstract
We have determined the nucleotide sequence of the rpoD gene which codes for the sigma subunit of RNA polymerase from E. coli K12. The gene, which we formerly cloned as a HindIII restriction fragment in the transducing phage, charon 25, was recloned into several plasmids. We have determined a 2600 base pair DNA sequence which includes the entire structural gene for sigma. The resulting amino acid sequence agrees with previous information obtained about sigma including the amino acid composition, partial sequence data for the N-terminus, the highly acidic nature of the polypeptide, and the cleavage pattern at cysteines. The molecular weight of 70,263 daltons calculated for the 613 amino acid polypeptides is significantly lower than had been determined previously by SDS polyacrylamide gel analysis.
摘要
我们已经确定了编码大肠杆菌K12 RNA聚合酶σ亚基的rpoD基因的核苷酸序列。该基因我们之前作为HindIII限制性片段克隆到转导噬菌体Charon 25中，现在又重新克隆到了几个质粒中。我们确定了一段2600个碱基对的DNA序列，其中包括σ的完整结构基因。所得的氨基酸序列与先前获得的关于σ的信息一致，包括氨基酸组成、N端的部分序列数据、多肽的高酸性性质以及半胱氨酸处的切割模式。为613个氨基酸的多肽计算出的分子量为70263道尔顿，明显低于先前通过SDS聚丙烯酰胺凝胶分析所确定的值。
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