Zheng H
Department of Infectious Diseases, Southwest Hospital, Third Military Medical College, Chongqing.
Zhonghua Yi Xue Za Zhi. 1993 May;73(5):273-5, 318.
A 27-peptide fragment derived from natural HDAg sequence was selected, identified and synthesized. It was used to develop an EIA method for detection of anti-HD. The 27-peptide possessed the similar antigenicity with the corresponding fragment of natural HDAg, and could also compete with natural HDAg for serum anti-HD. High specificity and no cross-reaction were found among the peptide and normal human sera, normal mice sera and sera positive with HAV, HBV or HCV antibody alone. 36 serum samples were identified previously, with Abbott kits with a coincident rate of 97.2%. Anti-HD was also detected in serum samples of some blood donors and patients with liver diseases and HBV infection from 1990-1992. One (0.33%) of 300 blood donors was positive (two times higher than normal ALT level in serum). 62 patients with hepatitis A and 58 patients with non-hepatitis B were anti-HD negative. 100 (11.64%) of 859 patients with HBV infection were positive: ASC 13/410 (3.17%), AH 7/63 (10.29%), CPH 1/9 (11.11%), CAH 22/121 (18.18%), SH 15/75 (20.00%), LC 23/78 (29.49%), PHC 19/89 (19.39%). The results are consistent with our previous reports.
从天然丁型肝炎病毒抗原（HDAg）序列中选取、鉴定并合成了一个27肽片段。该片段用于开发一种检测抗-HD的酶免疫分析（EIA）方法。此27肽与天然HDAg的相应片段具有相似的抗原性，并且还能与天然HDAg竞争血清中的抗-HD。该肽与正常人血清、正常小鼠血清以及仅抗甲型肝炎病毒（HAV）、乙型肝炎病毒（HBV）或丙型肝炎病毒（HCV）抗体阳性的血清之间无交叉反应，具有高度特异性。使用雅培试剂盒对36份血清样本进行预先鉴定，符合率为97.2%。还对1990 - 1992年期间一些献血者以及患有肝病和HBV感染患者的血清样本进行了抗-HD检测。300名献血者中有1名（0.33%）呈阳性（血清谷丙转氨酶水平比正常高两倍）。62例甲型肝炎患者和58例非乙型肝炎患者抗-HD呈阴性。859例HBV感染患者中有100例（11.64%）呈阳性：无症状携带者（ASC）13/410（3.17%），急性肝炎（AH）7/63（10.29%），慢性持续性肝炎（CPH）1/9（11.11%），慢性活动性肝炎（CAH）22/121（18.18%），肝硬化（SH）15/75（20.00%），肝癌（LC）23/78（29.49%），原发性肝癌（PHC）19/89（19.39%）。结果与我们之前的报告一致。
