去甲二氢愈创木酸（NDGA）对炔诺孕酮诱导的遗传毒性损伤的保护作用。

Protective effect of nordihydroguaiaretic acid (NDGA) against norgestrel induced genotoxic damage.

作者信息

Siddique Yasir Hasan, Beg Tanveer, Afzal Mohammad

机构信息

Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202002, Uttar Pradesh, India.

出版信息

Toxicol In Vitro. 2006 Mar;20(2):227-33. doi: 10.1016/j.tiv.2005.06.027. Epub 2005 Aug 2.

DOI:10.1016/j.tiv.2005.06.027

PMID:16061348

Abstract

Nordihydroguaiaretic acid (NDGA) is a phenolic lignan and possesses antioxidant and number of properties potentially useful to man. The effect of NDGA was studied against norgestrel induced genotoxic damage, using sister chromatid exchanges (SCEs), chromosomal aberrations (CAs), mitotic index (MI) and replication index (RI) as parameters. Amounts of 5, 10 and 20 microM of norgestrel was tested for its genotoxic effect in the absence as well as presence of S9 mix, and was found to be genotoxic at 10 and 20 microM in the presence of S9 mix. Again, 10 microM of norgestrel was treated with 0.5 and 1 microM of NDGA, separately, in the presence of S9 mix. Similar treatment was given with 20 microM of norgestrel. Treatments given with NDGA result in the reduction of SCE, CA and increase of MI as well as RI, suggesting its protective action on human lymphocytes in vitro against the norgestrel induced genotoxic damage.

摘要

去甲二氢愈创木酸（NDGA）是一种酚类木脂素，具有抗氧化作用以及许多对人类可能有用的特性。以姐妹染色单体交换（SCE）、染色体畸变（CA）、有丝分裂指数（MI）和复制指数（RI）为参数，研究了NDGA对炔诺孕酮诱导的遗传毒性损伤的影响。测试了5、10和20微摩尔炔诺孕酮在无S9混合物以及有S9混合物存在时的遗传毒性作用，发现在有S9混合物存在时，10和20微摩尔炔诺孕酮具有遗传毒性。同样，在有S9混合物存在的情况下，分别用0.5和1微摩尔NDGA处理10微摩尔炔诺孕酮。对20微摩尔炔诺孕酮进行了类似处理。用NDGA处理可导致SCE、CA减少，MI和RI增加，表明其在体外对人淋巴细胞免受炔诺孕酮诱导的遗传毒性损伤具有保护作用。