Tabara M, Watanabe M
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Chem Pharm Bull (Tokyo). 1992 Feb;40(2):449-51. doi: 10.1248/cpb.40.449.
Influences of indomethacin, which has been known as an inhibitor of the production of prostaglandins, on the suppression of footpad reaction (FPR) of BALB/c mice against sheep red blood cells by the painting of 12-O-tetradecanoylphorbol 13-acetate (TPA, a typical tumor promoter) were studied. The temporary suppressive effect by the painting of TPA (8 nmol) was abrogated by the painting of indomethacin (7-70 nmol) 60 min before TPA treatments. The lasting suppressive effect by TPA treatment (8 nmol/d) for 7 d following the painting of 7,12-dimethylbenz[a]anthracene (DMBA, 400 nmol), which is a typical tumor initiator, also disappeared when indomethacin (7 nmol) was painted 30-90 min before each TPA treatment. Influences of some inhibitors of tumor promotion on the lasting suppressive effect of FPR by DMBA and TPA were also tested. Painting of 0.6 mumol of 1-phenyl-2-pyrazolidone, 8.2 nmol of salcophytol A, 17 nmol of retinoic acid, 5.6 mumol of 3(2)-tert-butyl-4-hydroxyanisol, and 3 mumol of quercetin 45 min before each TPA treatment decreased the suppressive effect on the footpad reaction.
已知消炎痛是一种前列腺素生成抑制剂，本研究探讨了其对12-O-十四酰佛波醇-13-乙酸酯（TPA，一种典型的肿瘤促进剂）涂抹诱导的BALB/c小鼠对绵羊红细胞足垫反应（FPR）抑制作用的影响。在TPA处理前60分钟涂抹消炎痛（7 - 70 nmol）可消除TPA（8 nmol）涂抹所致的短暂抑制作用。在涂抹典型的肿瘤引发剂7,12-二甲基苯并[a]蒽（DMBA，400 nmol）后，TPA处理（8 nmol/天）持续7天所产生的持久抑制作用，在每次TPA处理前30 - 90分钟涂抹消炎痛（7 nmol）时也消失了。还测试了一些肿瘤促进抑制剂对DMBA和TPA诱导的FPR持久抑制作用的影响。在每次TPA处理前45分钟涂抹0.6 μmol的1-苯基-2-吡唑啉酮、8.2 nmol的水飞蓟宾A、17 nmol的视黄酸、5.6 μmol的3(2)-叔丁基-4-羟基茴香醚和3 μmol的槲皮素，可降低对足垫反应的抑制作用。
