Tabara M, Watanabe M
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Jpn J Exp Med. 1990 Dec;60(6):319-23.
In the preceding papers, we showed that the treatment of BALB/c mice with 12-O-tetradecanoylphorbol 13-acetate (TPA), a typical tumor promoter, suppressed footpad reaction (FPR) against sheep red blood cells (SRBC) temporarily but that this effect became lasting when TPA was administered to mice which had been treated with 7,12-dimethyl[a]-anthracene (DMBA), a typical tumor initiator, and that the effect of DMBA and TPA was caused by the induction of antigen-nonspecific T suppressor cells. In this work, we studied the effect of anthralin, a tumor promoter which has not the structure of phorbol ester, on FPR of BALB/c mice against SRBC. Painting of anthralin (80 nmol) suppressed FPR continuously (more than 7 days) unlike that of TPA. However, when anthralin was administered for 7 days following the treatment with 400 nmol of DMBA, the suppressive effect could be transferred with Thy-1 and Lyt-2 positive spleen cells whereas the suppressive effect by the painting of anthralin only for 7 days could not be transferred with the spleen cells.
在之前的论文中，我们表明，用典型的肿瘤促进剂12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）处理BALB/c小鼠，会暂时抑制其对绵羊红细胞（SRBC）的足垫反应（FPR），但当将TPA给予经典型肿瘤启动剂7,12 - 二甲基[a] - 蒽（DMBA）处理过的小鼠时，这种效应会持续存在，且DMBA和TPA的效应是由抗原非特异性T抑制细胞的诱导所引起的。在这项研究中，我们研究了没有佛波酯结构的肿瘤促进剂蒽林对BALB/c小鼠针对SRBC的FPR的影响。与TPA不同，涂抹蒽林（80 nmol）会持续抑制FPR（超过7天）。然而，在用400 nmol DMBA处理后给予蒽林7天，其抑制作用可通过Thy - 1和Lyt - 2阳性脾细胞转移，而仅涂抹蒽林7天的抑制作用则不能通过脾细胞转移。
