Tabara M, Watanabe M
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Jpn J Exp Med. 1988 Apr;58(2):67-72.
The effect of treatment with 12-tetradecanoylphorbol 13-acetate (TPA), a tumor promoter, following 7,12-dimethylbenz[a]anthracene (DMBA), a tumor iniator, on foodpad reaction (FPR) with sheep blood red cells (SRBC) in mice was studied. After painting of 400 nmol of DMBA on the skins of the BALB/c mice immunized with SRBC, 8 nmol of TPA was painted every day for 7 days. Then, FPR was measured 24 hr after 2nd injection of SRBC. In these experiments, FPR was markedly suppressed and this effect continued for 2 weeks. When the spleen cells from the unimmunized donor mice which had been treated with DMBA and TPA were injected into the recipient mice was suppressed. These data show that the suppressive effect on FPR may be caused by the increase of the suppressor cells which are not specific for the antigen. This effect disappeared by the treatment of the spleen cells with Thy-1 or Lyt-2 antibody. These results suggest that the suppressor cells has Thy-1 and Lyt-2 antigens.
研究了肿瘤启动剂7,12-二甲基苯并[a]蒽（DMBA）处理后，再用肿瘤促进剂12-十四酰佛波醇-13-乙酸酯（TPA）处理对小鼠用绵羊红细胞（SRBC）进行足垫反应（FPR）的影响。在用SRBC免疫的BALB/c小鼠皮肤上涂抹400 nmol的DMBA后，每天涂抹8 nmol的TPA，持续7天。然后，在第二次注射SRBC后24小时测量FPR。在这些实验中，FPR被显著抑制，且这种作用持续了2周。当将经DMBA和TPA处理的未免疫供体小鼠的脾细胞注射到受体小鼠中时，FPR受到抑制。这些数据表明，对FPR的抑制作用可能是由非抗原特异性的抑制细胞增加所致。用Thy-1或Lyt-2抗体处理脾细胞后，这种作用消失。这些结果提示，抑制细胞具有Thy-1和Lyt-2抗原。
