豌豆种子传播花叶马铃薯Y病毒RNA的5'非翻译区作为豌豆和烟草原生质体中的翻译增强子。

The 5' untranslated region from pea seedborne mosaic potyvirus RNA as a translational enhancer in pea and tobacco protoplasts.

作者信息

Nicolaisen M, Johansen E, Poulsen G B, Borkhardt B

机构信息

Danish Institute of Plant and Soil Science, Biotechnology Group, Lyngby.

出版信息

FEBS Lett. 1992 Jun 1;303(2-3):169-72. doi: 10.1016/0014-5793(92)80511-e.

DOI:10.1016/0014-5793(92)80511-e

PMID:1607015

Abstract

We have exploited the transient expression of foreign genes introduced into plant protoplasts to investigate the effect of the pea seedborne mosaic potyvirus (PSbMV) 5' untranslated region (5'UTR) on the level of gene expression in pea and tobacco protoplasts. The plant viral 5'UTRs were found to increase translation significantly in comparison to a plasmid containing no 5'UTR of viral origin. The enhancement effect of the 5'UTRs of PSbMV and tobacco etch potyvirus (TEV) was found to be similar in pea and tobacco protoplasts, indicating a host-independent role of the potyviral 5'UTRs in enhancing gene expression. Translational enhancement of the two potyviral 5'UTRs was similar to that of the 5'UTR of tobacco mosaic virus (TMV). This observation makes it attractive to use potyviral 5'UTRs as general translational enhancers in future genetic transformations of plants.

摘要

我们利用导入植物原生质体的外源基因的瞬时表达，来研究豌豆种传花叶马铃薯Y病毒（PSbMV）5'非翻译区（5'UTR）对豌豆和烟草原生质体中基因表达水平的影响。与不含病毒来源5'UTR的质粒相比，发现植物病毒5'UTR能显著增加翻译。发现PSbMV和烟草蚀纹马铃薯Y病毒（TEV）的5'UTR在豌豆和烟草原生质体中的增强作用相似，表明马铃薯Y病毒5'UTR在增强基因表达方面具有不依赖宿主的作用。两种马铃薯Y病毒5'UTR的翻译增强作用与烟草花叶病毒（TMV）的5'UTR相似。这一观察结果使得在未来的植物遗传转化中使用马铃薯Y病毒5'UTR作为通用翻译增强子具有吸引力。