Skotnicki M L, Ding S W, Mackenzie A M, Gibbs A J
Molecular Evolution and Systematics, Research School of Biological Sciences, Australian National University, Canberra.
Arch Virol. 1993;131(1-2):47-60. doi: 10.1007/BF01379079.
Eggplant mosaic virus (EMV) and ononis yellow mosaic virus (OYMV) are two tymoviruses that have ssRNA genomes of about 6.2 kb and 6.3 kb, and which infect solanaceous and leguminous hosts, respectively. Full-length cDNA clones of these viruses were constructed with a T7 promoter adjacent to the 5' terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3' terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated to Nicotiana glutinosa (for EMV) and Pisum sativum (for OYMV). These clones, together with clones of turnip yellow mosaic tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. These and other hybrids are being used to investigate the molecular basis for host range differences in tymoviruses.
茄子花叶病毒（EMV）和芒柄花黄色花叶病毒（OYMV）是两种番茄病毒，它们的单链RNA基因组分别约为6.2 kb和6.3 kb，分别感染茄科和豆科宿主。这些病毒的全长cDNA克隆是在病毒基因组DNA拷贝的5'末端附近构建一个T7启动子，并在3'末端构建独特的限制性内切酶位点。这使得能够从编码基因组的DNA转录RNA。当接种到黏烟草（针对EMV）和豌豆（针对OYMV）时，转录RNA具有感染性。这些克隆，连同感染十字花科植物的芜菁黄花叶番茄病毒的克隆，已被用于构建杂种，其中病毒粒子蛋白基因在EMV或OYMV与芜菁黄花叶病毒之间进行了交换。这些杂种和其他杂种正被用于研究番茄病毒宿主范围差异的分子基础。
