Teschner R, Postius S, Löffler M, Schneider F
Z Naturforsch C Biosci. 1979 Sep-Oct;34(9-10):805-10. doi: 10.1515/znc-1979-9-1024.
Cell proliferation, viability, DNA-, RNA-, protein synthesis, amino acid transport, repiration and lactate/glucose quotient of Ehrlich ascites tumor cells grown in suspension culture in serum free medium supplemented with albumin charges of different origin were studied. Optimal cell growth was obtained in nutrient medium supplemented with 1% bovine serum albumin (Cohn-fraction V, Serva). Cell proliferation under these culture conditions was delayed to 50% as compared to controls in normal medium; the rate of synthesis of macromolecules was reduced; energy metabolism was not significantly imparied. The trend of the cells in albumin medium to attach to glass was independent from the pH of the cultures between 7.2 and 8.0; it was enhanced by fatty acid deprivation of the albumin.
研究了在补充了不同来源白蛋白电荷的无血清培养基中悬浮培养的艾氏腹水癌细胞的细胞增殖、活力、DNA、RNA、蛋白质合成、氨基酸转运、呼吸作用以及乳酸/葡萄糖商。在补充1%牛血清白蛋白（Cohn-组分V，Serva）的营养培养基中获得了最佳细胞生长。与正常培养基中的对照相比，在这些培养条件下细胞增殖延迟至50%；大分子的合成速率降低；能量代谢没有明显受损。白蛋白培养基中细胞附着于玻璃的趋势与7.2至8.0之间的培养物pH无关；白蛋白的脂肪酸剥夺增强了这种趋势。
