Teschner R, Postius S, Löffler M, Schneider F
Z Naturforsch C Biosci. 1980 Jan-Feb;35(1-2):117-23. doi: 10.1515/znc-1980-1-222.
Cell proliferation, cell cycle distribution, viability, uptake of alpha-aminoisobutytic acid, cyclo leucine and 2-deoxyglucose as well as DNA, RNA and protein synthesis of Ehrlich ascites tumor cells grown in suspension culture over three passages in serum free nutrient medium supplemented with 1-2% bovine serum albumin) Cohn fraction V, Serva) were measured and compared to cells grown in medium supplemented with 15% horse serum. At the end of the fourth passage in vitro, that is the third passage in serum free albumin medium, the growth of the cells is reduced to about 10% of the controls, accumulation of the cells in G2 is delayed and the number of dead cells rises to 30%. The transport rates of alpha-aminoisobutyric acid and of 2-deoxyglucose slowly decrease while the uptake of cycloleucine shows a more complex behaviour in albumin medium. Incorporation of thymidine and leucine into the cells corresponds to the growth rate, while the incorporation of uridine in normal as well as in albumin medium exhibits different pattern. The present experiments demonstrate, that it may be possible to preserve this cells in a fairly "physiological" state during two passages in albumin medium.
对在添加了1 - 2%牛血清白蛋白（科恩V组分，塞尔瓦公司）的无血清营养培养基中悬浮培养三代的艾氏腹水瘤细胞的细胞增殖、细胞周期分布、活力、α-氨基异丁酸、环亮氨酸和2-脱氧葡萄糖的摄取以及DNA、RNA和蛋白质合成进行了测定，并与在添加15%马血清的培养基中生长的细胞进行了比较。在体外第四代培养结束时，即在无血清白蛋白培养基中的第三代培养时，细胞生长降至对照组的约10%，细胞在G2期的积累延迟，死细胞数量升至30%。α-氨基异丁酸和2-脱氧葡萄糖的转运速率缓慢下降，而环亮氨酸在白蛋白培养基中的摄取表现出更复杂的行为。胸腺嘧啶核苷和亮氨酸掺入细胞的情况与生长速率相对应，而尿苷在正常培养基和白蛋白培养基中的掺入表现出不同的模式。目前的实验表明，在白蛋白培养基中传代两代期间，有可能将这些细胞维持在相当“生理”的状态。
