甲氨蝶呤对烟曲霉菌株和黄曲霉菌株萌发及生长的影响。

Effect of methotrexate on the germination and growth of Aspergillus fumigatus and Aspergillus flavus strains.

作者信息

Warnock D W, Oliver D A, Cheung M M, Zurick N J

机构信息

Department of Microbiology, Bristol Royal Infirmary, UK.

出版信息

J Antimicrob Chemother. 1992 Apr;29(4):375-81. doi: 10.1093/jac/29.4.375.

DOI:10.1093/jac/29.4.375

PMID:1607326

Abstract

Methotrexate at concentrations of greater than or equal to 10(-5) M caused a significant reduction in the rate of germ tube formation of spores of Aspergillus fumigatus, but not of Aspergillus flavus, after 7 h incubation. At 10(-3) M the drug caused a significant reduction in germ tube elongation of A. fumigatus and A. flavus strains during the first 7 h of germination. After 18 h incubation, drug concentrations of greater than or equal to 4 x 10(-4) M produced greater than 80% reduction in growth of all strains tested. Potentiation of the antifungal effect against A. fumigatus was detected in tests in which methotrexate at 10(-4) M was used in combination with amphotericin B at 5 x 10(-7) M.

摘要

在孵育7小时后，浓度大于或等于10⁻⁵ M的甲氨蝶呤可显著降低烟曲霉孢子的芽管形成速率，但对黄曲霉孢子的芽管形成速率无显著影响。在10⁻³ M浓度下，该药物在萌发的前7小时可显著降低烟曲霉和黄曲霉菌株的芽管伸长。孵育18小时后，浓度大于或等于4×10⁻⁴ M的药物可使所有测试菌株的生长减少80%以上。在使用10⁻⁴ M甲氨蝶呤与5×10⁻⁷ M两性霉素B联合的测试中，检测到对烟曲霉的抗真菌作用增强。