El-Khoury Moufid, Ligot Rogine, Mahoney Simon, Stack Colin M, Perrone Gabriel G, Morton C Oliver
School of Science and Health, Western Sydney University, Building 21 Campbelltown Campus, Narellan Road, Campbelltown, NSW, 2560, Australia.
School of Science and Health, Western Sydney University, Locked Bag 1797, Penrith, NSW, 2571, Australia.
BMC Res Notes. 2017 Aug 1;10(1):364. doi: 10.1186/s13104-017-2696-4.
Recent studies into the antifungal activity of NK-cells against the Aspergillus fumigatus have presented differing accounts on their mode of antifungal activity. One of these mechanisms proposed that NK-cells may kill the fungus via the direct effects of exposure to Interferon gamma (IFN-γ).
In this study we investigated the direct antifungal effects of recombinant human IFN-γ against a range of pathogenic fungi by measuring cellular damage using an XTT-based assay and cell viability through plate counts. It was found that 32 pg/ml of IFN-γ exhibited a significant but small antifungal effect on A. fumigatus (p = 0.02), Aspergillus flavus (p = 0.04) and Saccharomyces cerevisiae (p = 0.03), inhibiting growth by 6, 11 and 17% respectively. No significant inhibitory effects were observed in Candida species (p > 0.05 for all species tested) or Cryptococus neoformans (p = 0.98). Short term exposure (3 h) to a combination of amphotericin B (1 µg/ml) and IFN-γ (32 pg/ml) increased the effectiveness of amphotericin B against A. fumigatus and S. cerevisiae but not Candida albicans. These data suggest that IFN-γ does not possess strong antifungal activity but can enhance the effect of amphotericin B under some testing conditions against Aspergillus species.
近期关于自然杀伤细胞（NK 细胞）抗烟曲霉真菌活性的研究，对其抗真菌活性模式给出了不同的描述。其中一种机制认为，NK 细胞可能通过暴露于干扰素γ（IFN -γ）的直接作用来杀死真菌。
在本研究中，我们通过基于 XTT 的检测方法测量细胞损伤，并通过平板计数来检测细胞活力，从而研究重组人 IFN -γ对一系列致病真菌的直接抗真菌作用。结果发现，32 pg/ml 的 IFN -γ对烟曲霉（p = 0.02）、黄曲霉（p = 0.04）和酿酒酵母（p = 0.03）表现出显著但较小的抗真菌作用，分别抑制其生长 6%、11%和 17%。在念珠菌属（所有测试菌种 p > 0.05）或新型隐球菌（p = 0.98）中未观察到显著的抑制作用。短期暴露（3 小时）于两性霉素 B（1 μg/ml）和 IFN -γ（32 pg/ml）的组合，可增强两性霉素 B 对烟曲霉和酿酒酵母的作用，但对白色念珠菌无效。这些数据表明，IFN -γ不具有强大的抗真菌活性，但在某些测试条件下可增强两性霉素 B 对曲霉属菌种的作用。
