三七总皂苷对阿霉素诱导心肌损伤的保护作用研究

[Study on protective effects of Panax notoginseng saponins on doxorubicin-induced myocardial damage].

作者信息

Shi Run, Liu Li, Huo Yang, Cheng Yi-Yu

机构信息

Zhejiang University, College of Pharmaceutical Sciences, Department of Sciences and Engineering of Traditional Chinese Medicines, Hangzhou 310058, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 Dec;32(24):2632-5.

PMID:18338604

Abstract

OBJECTIVE

To study the effects of Panax notoginseng saponins (PNS) on acute doxorubicin-induced myocardial injury in mice and the anti-tumor efficiency of doxorubicin.

METHOD

Mice were given a dose of 15 mg x kg(-1) doxorubicin ip alone or in combination with 25, 50, 100 mg x kg(-1) PNS ig, 5 days before doxorubicin administration and following 3 days. Cardiotoxic effects were measured by serum levels of dehydrogenase (LDH), creatine kinase (CK) and creatine kinase isoenzyme (CK-MB) and activities of antioxidant enzymes in heart tissue. In vitro experiments were performed using embryonic rat heart cell H9C2 to assess the protective effect of PNS (6.25-100 mg x L(-1)) against doxorubicin on cell viability. Anti-tumor efficiency of doxorubicin was evaluated by cytotoxic experiments using three cancer cell lines.

RESULT

Pretreatment with PNS significantly lowered the levels of serum LDH, CK and CK-MB, and normalized myocardial superoxide dismutase, glutathione peroxidase and catalase activities. PNS also attenuated the inhibitory effect of doxorubicin on the viability of H9C2 cells, but did not compromise its inhibitory effect on proliferation of cancer cells.

CONCLUSION

PNS was demonstrated to attenuate doxorubicin-induced myocardial damage without compromising its anti-tumor activity.

摘要

目的

研究三七总皂苷（PNS）对阿霉素诱导的小鼠急性心肌损伤的影响及阿霉素的抗肿瘤效果。

方法

在给予阿霉素前5天及之后3天，小鼠单独腹腔注射15 mg·kg⁻¹阿霉素，或联合灌胃25、50、100 mg·kg⁻¹ PNS。通过检测血清中脱氢酶（LDH）、肌酸激酶（CK）和肌酸激酶同工酶（CK-MB）水平以及心脏组织中抗氧化酶活性来评估心脏毒性作用。使用胚胎大鼠心脏细胞H9C2进行体外实验，以评估PNS（6.25 - 100 mg·L⁻¹）对阿霉素所致细胞活力的保护作用。通过对三种癌细胞系进行细胞毒性实验来评估阿霉素的抗肿瘤效果。