Waseem Amir, Yaqoob Mohammad, Nabi Abdul
Department of Chemistry, University of Balochistan, Quetta, Pakistan.
Anal Sci. 2006 Aug;22(8):1095-8. doi: 10.2116/analsci.22.1095.
A flow-injection method is reported for the determination of thyroxine based on its enhancement effect on the tris(2,2'-bipyridyl)ruthenium(III) chemiluminescence reaction in the presence of NADH using immobilized alcohol dehydrogenase purified from baker yeast. The limit of detection (3 sigma blank) was 5.0 x 10(-8) M with a sample throughput of 80 h(-1). The calibration graph was linear over the range 0.5 - 10 x 10(-7) M (r2= 0.9988) with the relative standard deviation in the range 1.4 - 3.5% (n = 4). The effect of common excipients used in pharmaceutical preparations, some organic compounds and metal ions was studied. The method was applied to pharmaceutical thyroxine tablets, and the obtained results were not significantly different from the amount quoted.
报道了一种流动注射法，用于测定甲状腺素。该方法基于在使用从面包酵母中纯化的固定化乙醇脱氢酶的情况下，甲状腺素对三（2,2'-联吡啶）钌（III）化学发光反应的增强作用。检测限（3σ空白）为5.0×10⁻⁸ M，样品通量为80 h⁻¹。校准曲线在0.5 - 10×10⁻⁷ M范围内呈线性（r² = 0.9988），相对标准偏差在1.4 - 3.5%范围内（n = 4）。研究了药物制剂中常用辅料、一些有机化合物和金属离子的影响。该方法应用于药物甲状腺素片，所得结果与标注量无显著差异。
