Skrzypek T, Valverde Piedra J L, Skrzypek H, Woliński J, Kazimierczak W, Szymańczyk S, Pawłowska M, Zabielski R
Department of Animal Biochemistry and Physiology, Faculty of Veterinary Medicine, Agriculture University in Lublin, Lublin, Poland.
J Physiol Pharmacol. 2005 Jun;56 Suppl 3:71-87.
Modifications in the structure of gastrointestinal mucosa is often used to evaluate gut function for instance during the development or in response to particular food components. Scanning electron microscopy (SEM) gives a chance to observe the surface of the gut epithelium in three dimensions. However, this technique is seldom used due to technical difficulties. The present study attempted to investigate the intestinal mucosa structure changes in the postnatal pig using light and scanning electron microscopy technique. Experiments were carried out on sow reared piglets from birth until 38 days of age. Piglets were sacrificed at birth and at the 3(rd), 7(th), 21(st) and 38(th) day of life. The entire gastrointestinal tract was immediately harvested and the whole thickness tissue samples were taken from the duodenum, jejunum and ileum for optical and scanning electron microscopy. SEM analyses corroborated with histometry made by optical microscopy. Moreover, a number of shape modifications of the villi and its surface have been observed. The development changes in small intestine mucosa during the first 3 weeks were manifested in shape, size and density of villi. In conclusion, the structure of small intestinal mucosa undergoes profound structural changes. SEM gives a new dimension in the investigation of gut mucosa.
胃肠道黏膜结构的改变常被用于评估肠道功能，例如在发育过程中或对特定食物成分的反应中。扫描电子显微镜（SEM）能够从三维角度观察肠道上皮表面。然而，由于技术难题，该技术很少被使用。本研究试图运用光学显微镜和扫描电子显微镜技术，研究出生后仔猪的肠黏膜结构变化。实验选用母猪饲养的仔猪，从出生至38日龄。仔猪在出生时以及出生后第3、7、21和38天被处死。立即采集整个胃肠道，并从十二指肠、空肠和回肠获取全层组织样本，用于光学显微镜和扫描电子显微镜检查。扫描电子显微镜分析结果与光学显微镜组织测量结果相符。此外，还观察到绒毛及其表面的一些形态变化。前三周小肠黏膜的发育变化体现在绒毛的形状、大小和密度上。总之，小肠黏膜结构经历了深刻的结构变化。扫描电子显微镜为肠道黏膜的研究提供了新的维度。
