用铽敏化电致化学发光法测定诺氟沙星。

Determination of norfloxacin using a terbium-sensitized electrogenerated chemiluminescence method.

作者信息

Chen Shi-Lv, Liu Yu, Zhao Hui-Chun, Jin Lin-Pei, Zhang Zhong-Lun, Zheng Yan-Zhen

机构信息

Department of Chemistry, Beijing Normal University, Beijing 100875, People's Republic of China.

出版信息

Luminescence. 2006 Jan-Feb;21(1):20-5. doi: 10.1002/bio.876.

DOI:10.1002/bio.876

PMID:16078306

Abstract

A simple electrogenerated chemiluminescence (ECL) analysis method for the determination of norfloxacin (NFLX) is reported. It is based on ECL produced by Na(2)SO(3), which is sensitized by the Tb-NFLX complex. The relative ECL intensity of the Tb(3+)-NFLX-Na(2)SO(3) system is proportional to the amount of NFLX. The optimized experimental conditions were investigated. The linear range and detection limit for NFLX were 1.0 x 10(-10)-8.0 x 10(-7) mol/L and 2.8 x 10(-11) mol/L, respectively. This method was successfully applied to the determination of NFLX in a capsule. NFLX in urine can be directly detected without pretreatment or separation.

摘要

报道了一种用于测定诺氟沙星（NFLX）的简单的电化学发光（ECL）分析方法。该方法基于亚硫酸钠产生的ECL，其由Tb-NFLX络合物敏化。Tb(3+)-NFLX-Na(2)SO(3)体系的相对ECL强度与NFLX的量成正比。研究了优化的实验条件。NFLX的线性范围和检测限分别为1.0×10(-10)-8.0×10(-7) mol/L和2.8×10(-11) mol/L。该方法成功应用于胶囊中NFLX的测定。尿液中的NFLX无需预处理或分离即可直接检测。