Nakazono Takehiko, Kashimura Seiichi, Hayashiba Yasuhiko, Hara Kenji, Miyoshi Aya
Department of Forensic Medicine, Fukuoka University School of Medicine, 7-45-1, Nanakuma, Jonan-ku, Fukuoka, 814-0180, Japan.
J Forensic Sci. 2005 Jul;50(4):860-4.
To evaluate the utility of DNA polymorphism typing of urine stains in forensic investigations, the amplifiable amount of DNA was estimated in 20 urine specimens obtained from 10 male and 10 female volunteers using a DNA purification kit following dialfiltration. DNA obtained from both urine and urine stains was amplified with the AmpflSTR Profiler PCR Amplification Kit, and was analyzed by capillary electrophoresis using the Genetic Analyzer. The amount of male and female urine necessary for obtaining a complete DNA profile was 0.2 mL and 0.08 mL, respectively. When 0.2 mL of male urine were used to create urine stains, complete DNA profiles could be obtained from just some of the stains. However, when only 0.1 mL of female urine was used, complete profiles could be successfully obtained from all of the stains. DNA on bleached cotton remained amplifiable for 3-6 weeks. This method using a DNA purification kit following dialfiltration can be recommended for the genotyping of urine stains.
为评估尿渍DNA多态性分型在法医调查中的实用性，使用透析过滤后的DNA纯化试剂盒，对从10名男性和10名女性志愿者获取的20份尿液标本中的可扩增DNA量进行了估计。从尿液和尿渍中获得的DNA用AmpflSTR Profiler PCR扩增试剂盒进行扩增，并使用遗传分析仪通过毛细管电泳进行分析。获得完整DNA图谱所需的男性和女性尿液量分别为0.2 mL和0.08 mL。当用0.2 mL男性尿液制作尿渍时，仅从部分尿渍中就能获得完整的DNA图谱。然而，当仅使用0.1 mL女性尿液时，所有尿渍均能成功获得完整图谱。漂白棉上的DNA在3 - 6周内仍可扩增。这种透析过滤后使用DNA纯化试剂盒的方法可推荐用于尿渍的基因分型。
