尘肺病煤矿工人的氧化损伤与血清细胞因子
[Oxidative injury and serum cytokines in coal workers with pneumoconiosis].
作者信息
Yao Wu, Wang Zhi-ming, Wang Mian-zhen, Wang Na
机构信息
Department of Occupational Medicine, West China School of Public Health, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Jul;36(4):510-2.
OBJECTIVE
To inquire into the implication of oxidative injury and two cytokines TGF-beta1 and FGF in the occurrence and development of coal worker's Pneumoconiosis.
METHODS
The methods of nitrate reductase, TBA, and xanthine oxidase were used to detect the levels of serum NO, MDA and SOD respectively in 50 patients suffering from coal worker's Pneumoconiosis and 50 healthy controls, then ELISA was performed to detect the level of TGF-beta1 and FGF in the two groups.
RESULTS
The levels of NO and MDA in serum of patients with coal worker's Pneumoconiosis were higher than those in the controls; the level of SOD was lower in the patients than in the controls; and there were statistically significant differences between the two groups (P<0.05). Statistically significant differences in TGF-beta1 and FGF were also noted between the two group.
CONCLUSION
In this study, the patients with coal worker's Pneumoconiosis were found to have abnormity of oxyradical reaction, unblanced oxidation/antioxidation state, and abnormally high expression of cytokine, which might have relations with the occurrence and development of coal worker's Pneumoconiosis.
目的
探讨氧化损伤及两种细胞因子转化生长因子β1（TGF-β1）和成纤维细胞生长因子（FGF）在煤工尘肺发生发展中的意义。
方法
采用硝酸还原酶法、硫代巴比妥酸法和黄嘌呤氧化酶法分别检测50例煤工尘肺患者和50例健康对照者血清一氧化氮（NO）、丙二醛（MDA）和超氧化物歧化酶（SOD）水平，然后用酶联免疫吸附测定法（ELISA）检测两组患者TGF-β1和FGF水平。
结果
煤工尘肺患者血清NO和MDA水平高于对照组；患者SOD水平低于对照组；两组间差异有统计学意义（P<0.05）。两组间TGF-β1和FGF也有统计学意义上的差异。
结论
本研究发现煤工尘肺患者存在氧自由基反应异常、氧化/抗氧化状态失衡及细胞因子表达异常增高，这可能与煤工尘肺的发生发展有关。
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