Goto C, Minobe Y, Iizuka T
National Agriculture Research Center, Ibaraki, Japan.
J Gen Virol. 1992 Jun;73 ( Pt 6):1491-7. doi: 10.1099/0022-1317-73-6-1491.
Restriction endonuclease analysis was performed on the genomic DNA of granulosis viruses isolated from noctuid species of six genera: Xestia c-nigrum, Autographa gamma, Hydraecia amurensis, Celaena leucostigma, Aletia pallens and Pseudaletia separata. All of the isolates gave very similar restriction endonuclease profiles with only minor variations. An isolate obtained from X. c-nigrum was chosen as the reference genotype, and a genomic library was constructed for this isolate using plasmid vectors. The genome was mapped using EcoRI, BamHI and BglII, and Southern hybridization; the size of the genome was estimated to be 179 kbp. Hybridization of labelled clones to fragments of other isolates revealed that genotypic variation among isolates resulted from changes in restriction sites, and from deletion or insertion of DNA. Comparative restriction mapping revealed that all of the isolates were variants of one virus, even though they originated from different host species.
对从六个属的夜蛾科物种中分离出的颗粒体病毒的基因组DNA进行了限制性内切酶分析，这六个属分别是：八字地老虎、苜蓿银纹夜蛾、黑龙江冬夜蛾、白纹夜蛾、苍白艾夜蛾和粘虫。所有分离株的限制性内切酶图谱非常相似，只有微小差异。从八字地老虎中获得的一个分离株被选为参考基因型，并使用质粒载体为该分离株构建了基因组文库。使用EcoRI、BamHI和BglII以及Southern杂交对基因组进行了图谱绘制；估计基因组大小为179 kbp。标记克隆与其他分离株片段的杂交表明，分离株之间的基因型变异是由限制性位点的变化以及DNA的缺失或插入引起的。比较限制性图谱分析表明，所有分离株都是一种病毒的变体，尽管它们源自不同的宿主物种。
