Zhang Qing-Ping, Wang Xiu-E, Wang Yao-Nan, Zhao Yan, Wang Hai-Yan, Wang Su-Ling, Chen Pei-Du
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute of Nanjing Agricultural University, Nanjing 210095, China.
Yi Chuan Xue Bao. 2005 Jul;32(7):733-7.
Three wheat spindle streak mosaic viruses (WSSMV) resistant cultivars ('Yining Xiaomai', 'Xu87-633', and 'Xifeng') and one susceptible cultivar ('Zhen9523') were used as parents of 3 crosses in this experiment. WSSMV resistance of the parents, F1, and F2 was evaluated under field condition. Based on the segregation ratios of resistant and susceptible plants in F, and F2 populations, it was deduced that the resistance to WSSMV was dominant and the inheritable factors controlling WSSMV resistance were encoded by the nuclear genome. WSSMV resistances in 'Yining Xiaomai' and 'Xifeng' were controlled by two pairs of alleles, which showed complementary effects. However the resistance in 'Xu-87633' was controlled by a single dominant gene. 266 pairs of SSR primers located on 21 wheat chromosomes were used for polymorphic analysis of the two resistant and the susceptible parents 'Yining Xiaomai' and 'Zhen9523', and 108 of them amplified polymorphic DNA products. By Bulk Segregant Analysis of resistant and susceptible pools, one pair of primer located on chromosome arm 2DS, Xgwm261, were found being linked to WSSMV resistance. The 224 F2 individuals were then amplified with marker Xgwm261. The statistic genetic distance between Xgwm261 and the resistance locus was calculated to be 22.9 cM using the software Mapmaker 3.0.
本试验选用3个抗小麦梭条斑花叶病毒（WSSMV）品种（‘伊宁小麦’‘许87-633’和‘西峰’）和1个感病品种（‘镇9523’）作为3个杂交组合的亲本。在田间条件下对亲本、F1和F2的WSSMV抗性进行了评价。根据F1和F2群体中抗病和感病植株的分离比例，推断对WSSMV的抗性为显性，控制WSSMV抗性的遗传因子由核基因组编码。‘伊宁小麦’和‘西峰’的WSSMV抗性由两对等位基因控制，表现出互补效应。而‘许87633’的抗性由一个显性单基因控制。利用位于21条小麦染色体上的266对SSR引物，对两个抗病亲本‘伊宁小麦’和感病亲本‘镇9523’进行多态性分析，其中108对引物扩增出多态性DNA产物。通过对抗病池和感病池的混合分离分析，发现位于2DS染色体臂上的一对引物Xgwm261与WSSMV抗性连锁。然后用标记Xgwm261对224个F2个体进行扩增。使用Mapmaker 3.0软件计算出Xgwm261与抗性基因座之间的统计遗传距离为22.9 cM。
