[将圆柱山羊草的抗散黑穗病基因导入普通小麦的分子标记定位]

[Molecular marker mapping of the gene resistant to common bunt transferred from Aegilops cylindrica into bread wheat].

作者信息

Galaev A V, Babaiants L T, Sivolap Iu M

出版信息

Tsitol Genet. 2006 Mar-Apr;40(2):3-11.

Abstract

Introgression lines 5/55-91 and 378/2000 of bread wheat contain the gene of resistance to Tilletia caries (DC.) Tul. transferred from Aegilops cylindrica Host. Using bulked segregant analysis with ISSR and SSR PCR the lincage of microsatellite locus Xgwm 259 with the gene of common bunt resistance has been identified in F2 population of 378/2000 x Lutestens 23397. DNA mapping made it possible to localize this highly effective gene in the intercalary region of the long arm of wheat chromosome 1B at the distance of 7.6-8.5 cM of the microsatellite Xgwm 259 locus which thus can be used in wheat breeding for selection of genotype resistance to common bunt.

摘要

普通小麦的渗入系5/55 - 91和378/2000含有从圆柱山羊草导入的抗小麦网腥黑穗病菌（DC.）Tul.的基因。利用ISSR和SSR PCR的混合分离群体分析法，在378/2000×Lutestens 23397的F2群体中鉴定出微卫星位点Xgwm 259与抗网腥黑穗病基因的连锁关系。DNA图谱绘制使得将这个高效基因定位在小麦1B染色体长臂的居间区域成为可能，该区域距微卫星Xgwm 259位点7.6 - 8.5厘摩，因此可用于小麦育种中对网腥黑穗病抗性基因型的选择。

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