Kabir Md Shahinur, Yamashita Daisuke, Koyama Satoshi, Oshima Taku, Kurokawa Ken, Maeda Maki, Tsunedomi Ryouichi, Murata Masayuki, Wada Chieko, Mori Hirotada, Yamada Mamoru
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
Graduate School of Information Science, Nara Institute of Science and Technology, Nara 630-01, Japan.
Microbiology (Reading). 2005 Aug;151(Pt 8):2721-2735. doi: 10.1099/mic.0.28004-0.
It has been shown that Escherichia coli cells with increased expression of the rpoE gene encoding sigma(E) exhibit enhanced cell lysis in early stationary phase. Further analysis of the lysis phenomenon was performed using a transient expression system of the rpoE gene and by DNA microarray. The former analysis revealed a sigma(E)-directed cell lysis, specific for early stationary phase but not for the exponential phase. The microarray analysis with RNAs from exponential and early stationary phase cells revealed that a large number of genes were up- or down-regulated when the rpoE gene was induced, and that several genes were induced in a phase-specific manner. The upregulated genes include many previously identified sigma(E) regulon genes, suggesting that a large number of genes are under the control of sigma(E) in this organism. These genes are involved in various cellular activities, including the cell envelope, cellular processes, regulatory functions, transport and translation. Genes that are presumably related to phase-specific cell lysis in E. coli are discussed.
已表明，编码σ（E）的rpoE基因表达增加的大肠杆菌细胞在稳定期早期表现出增强的细胞裂解。使用rpoE基因的瞬时表达系统并通过DNA微阵列对裂解现象进行了进一步分析。前一项分析揭示了一种由σ（E）介导的细胞裂解，这种裂解对稳定期早期具有特异性，而对指数期则不具有特异性。对指数期和稳定期早期细胞的RNA进行微阵列分析表明，当rpoE基因被诱导时，大量基因被上调或下调，并且一些基因以阶段特异性方式被诱导。上调的基因包括许多先前鉴定的σ（E）调控子基因，这表明在这种生物体中大量基因受σ（E）的控制。这些基因参与各种细胞活动，包括细胞壁、细胞过程、调节功能、转运和翻译。讨论了可能与大肠杆菌阶段特异性细胞裂解相关的基因。
