σ(E) 调控子在稳定期大肠杆菌死细胞裂解中的作用。
Function of the sigma(E) regulon in dead-cell lysis in stationary-phase Escherichia coli.
作者信息
Nitta T, Nagamitsu H, Murata M, Izu H, Yamada M
机构信息
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
出版信息
J Bacteriol. 2000 Sep;182(18):5231-7. doi: 10.1128/JB.182.18.5231-5237.2000.
Abstract
Elevation of active sigma(E) levels in Escherichia coli by either repressing the expression of rseA encoding an anti-sigma(E) factor or cloning rpoE in a multicopy plasmid, led to a large decrease in the number of dead cells and the accumulation of cellular proteins in the medium in the stationary phase. The numbers of CFU, however, were nearly the same as those of the wild type or cells devoid of the cloned gene. In the wild-type cells, rpoE expression was increased in the stationary phase and a low-level release of intracellular proteins was observed. These results suggest that dead cell lysis in stationary-phase E. coli occurs in a sigma(E)-dependent fashion. We propose there is a novel physiological function of the sigma(E) regulon that may guarantee cell survival in prolonged stationary phase by providing nutrients from dead cells for the next generation.
摘要
通过抑制编码抗σ（E）因子的rseA的表达或在多拷贝质粒中克隆rpoE来提高大肠杆菌中活性σ（E）水平，导致稳定期死细胞数量大幅减少以及培养基中细胞蛋白的积累。然而，菌落形成单位（CFU）的数量与野生型或缺失克隆基因的细胞几乎相同。在野生型细胞中，rpoE表达在稳定期增加，并且观察到细胞内蛋白质的低水平释放。这些结果表明，稳定期大肠杆菌中的死细胞裂解以σ（E）依赖性方式发生。我们提出，σ（E）调节子具有一种新的生理功能，即通过为下一代提供来自死细胞的营养物质，保证细胞在延长的稳定期内存活。
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