Metori Koichi, Sei Yoshihisa, Kimura Yumiko, Ozawa Tomoyuki, Yamaguchi Kentaro, Miyake Muneharu
College of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi, Chiba 274-8555, Japan.
Chem Pharm Bull (Tokyo). 2005 Aug;53(8):1029-33. doi: 10.1248/cpb.53.1029.
The investigation of the host-guest complex formations between cyclophane (TGDMAP) (1) as a host and L-acidic amino acids such as L-glutamic acid (Glu) and L-aspartic acid (Asp) as guests was carried out using fast atom bombardment (FAB), electrospray ionization (ESI) and cold-spray ionization (CSI) mass spectrometry (MS). The stability constant (K(s)) values obtained by the three different MS methods almost agreed. However, the complex ion peaks of a novel cyclophane (CPCn) (2) with Glu and Asp were not observed in FAB-MS. Then, these host-guest complex formations by use of CSI-MS and ESI-MS was examined, as the results, these complex ion peaks were observed clearly and the measurement values by the two MS methods are mostly in agreement. It was concluded that ESI-MS and CSI-MS are available for the determination of K(s) value as well as FAB-MS.
以环番（TGDMAP）（1）为主客体，L-谷氨酸（Glu）和L-天冬氨酸（Asp）等L-酸性氨基酸为客体，采用快原子轰击（FAB）、电喷雾电离（ESI）和冷喷雾电离（CSI）质谱（MS）对主客体配合物的形成进行了研究。通过三种不同的质谱方法获得的稳定常数（K(s)）值几乎一致。然而，在FAB-MS中未观察到新型环番（CPCn）（2）与Glu和Asp的络合离子峰。然后，利用CSI-MS和ESI-MS对这些主客体配合物的形成进行了研究，结果清楚地观察到了这些络合离子峰，并且两种质谱方法的测量值大多一致。得出结论，ESI-MS和CSI-MS与FAB-MS一样可用于K(s)值的测定。
