Tomer K B, Perkins J R, Parker C E, Deterding L J
Laboratory of Molecular Biophysics, National Institute for Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Biol Mass Spectrom. 1991 Dec;20(12):783-8. doi: 10.1002/bms.1200201207.
A comparison of coaxial continuous flow fast atom bombardment (FAB) with static FAB and with electrospray ionization (ESI) for the analysis of 'high'-mass peptides (Mr = 3000-4000) is presented. Sensitivities of the peptides by coaxial continuous flow FAB is nearly an order of magnitude better than by static FAB. Single-scan spectra with good signal-to-noise can be obtained from as little as 200 fmol (by flow injection analysis). Detection limits by ESI mass spectrometry were found to be equivalent to 20 times higher than by coaxial continuous flow FAB on a per mole basis, but 4-20 times lower on a concentration basis, owing to the greater flow per unit time employed in the ESI mass spectrometric experiments.
本文介绍了同轴连续流快原子轰击（FAB）与静态FAB以及电喷雾电离（ESI）在分析“高”质量肽（Mr = 3000 - 4000）方面的比较。同轴连续流FAB对肽的灵敏度比静态FAB几乎高一个数量级。通过流动注射分析，仅200 fmol就能获得具有良好信噪比的单扫描光谱。发现ESI质谱法的检测限按每摩尔计算比同轴连续流FAB高20倍，但按浓度计算低4 - 20倍，这是由于ESI质谱实验中单位时间内的流量更大。
