Akgöl Sinan, Bereli Nilay, Denizli Adil
Department of Chemistry, Biochemistry Division, Adnan Menderes University, Aydin, Turkey.
Macromol Biosci. 2005 Aug 12;5(8):786-94. doi: 10.1002/mabi.200400230.
Casein is well known as a good protein emulsifier and beta-casein is the major component of casein and commercial sodium caseinate. Dye affinity adsorption is increasingly used for protein separation. beta-Casein adsorption onto Reactive Red 120 attached magnetic poly(2-hydroxyethyl methacrylate) (m-PHEMA) beads was investigated in this work. m-PHEMA beads (80-120 microm in diameter) were produced by dispersion polymerization. The dichlorotriazine dye Reactive Red 120 was attached covalently as a ligand. The dye attached beads, having a swelling ratio of 55% (w/w) and carrying different amounts of Reactive Red 120 (9.2 micromol . g(-1)-39.8 micromol . g(-1)), were used in beta-casein adsorption studies. The effects of the initial concentration, pH, ionic strength and temperature on the adsorption efficiency of dye attached beads were studied in a batch reactor. The non-specific adsorption on the m-PHEMA beads was 1.4 mg . g(-1). Reactive Red 120 attachment significantly increased the beta-casein adsorption up to 37.3 mg . g(-1). More than 95.4% of the adsorbed beta-casein was desorbed in 1 h in a desorption medium containing 1.0 M KSCN at pH 8.0. We concluded that Reactive Red 120 attached m-PHEMA beads can be applied for beta-casein adsorption without significant losses in the adsorption capacities.
酪蛋白是一种众所周知的优质蛋白质乳化剂，而β-酪蛋白是酪蛋白和商业酪蛋白酸钠的主要成分。染料亲和吸附越来越多地用于蛋白质分离。本研究考察了β-酪蛋白在附着有活性红120的磁性聚甲基丙烯酸2-羟乙酯（m-PHEMA）微球上的吸附情况。m-PHEMA微球（直径80-120微米）通过分散聚合制备。二氯三嗪染料活性红120作为配体共价连接。将溶胀率为55%（w/w）且负载不同量活性红120（9.2微摩尔·克⁻¹-39.8微摩尔·克⁻¹）的染料连接微球用于β-酪蛋白吸附研究。在间歇反应器中研究了初始浓度、pH值、离子强度和温度对染料连接微球吸附效率的影响。m-PHEMA微球上的非特异性吸附为1.4毫克·克⁻¹。活性红120的连接显著提高了β-酪蛋白的吸附量，最高可达37.3毫克·克⁻¹。在pH 8.0、含有1.0 M KSCN的解吸介质中，1小时内超过95.4%吸附的β-酪蛋白被解吸。我们得出结论，附着有活性红120的m-PHEMA微球可用于β-酪蛋白吸附，且吸附容量不会有显著损失。
