Kuru Cansu İlke, Ulucan-Karnak Fulden, Dayıoğlu Büşra, Şahinler Mert, Şendemir Aylin, Akgöl Sinan
Department of Biochemistry, Faculty of Science, Ege University, 35100 İzmir, Turkey.
Department of Bioengineering, Faculty of Engineering, Ege University, 35100 İzmir, Turkey.
Polymers (Basel). 2024 Jan 9;16(2):196. doi: 10.3390/polym16020196.
Cancer is still the leading cause of death in the world despite the developing research and treatment opportunities. Failure of these treatments is generally associated with cancer stem cells (CSCs), which cause metastasis and are defined by their resistance to radio- and chemotherapy. Although known stem cell isolation methods are not sufficient for CSC isolation, they also bring a burden in terms of cost. The aim of this study is to develop a high-efficiency, low-cost, specific method for cancer stem cell isolation with magnetic functional nanoparticles. This study, unlike the stem cell isolation techniques (MACS, FACS) used today, was aimed to isolate cancer stem cells (separation of CD133 cells) with nanoparticles with specific affinity and modification properties. For this purpose, affinity-based magnetic nanoparticles were synthesized and characterized by providing surface activity and chemical reactivity, as well as making surface modifications necessary for both lectin affinity and metal affinity interactions. In the other part of the study, synthesized and characterized functional polymeric magnetic nanoparticles were used for the isolation of CSC from the human osteosarcoma cancer cell line (SAOS-2) with a cancer stem cell subpopulation bearing the CD133 surface marker. The success and efficiency of separation after stem cell isolation were evaluated via the MACS and FACS methods. As a result, when the His-graft-mg-p(HEMA) nanoparticle was used at a concentration of 0.1 µg/mL for 10 and 10 cells, superior separation efficiency to commercial microbeads was obtained.
尽管有不断发展的研究和治疗机会，但癌症仍是全球主要的死亡原因。这些治疗方法的失败通常与癌症干细胞（CSC）有关，癌症干细胞会导致转移，并且其对放疗和化疗具有抗性。虽然已知的干细胞分离方法不足以用于分离癌症干细胞，但它们在成本方面也带来了负担。本研究的目的是开发一种利用磁性功能纳米颗粒高效、低成本、特异性分离癌症干细胞的方法。与当今使用的干细胞分离技术（磁珠分选法、荧光激活细胞分选法）不同，本研究旨在利用具有特定亲和力和修饰特性的纳米颗粒分离癌症干细胞（分离CD133细胞）。为此，通过提供表面活性和化学反应性，以及进行凝集素亲和力和金属亲和力相互作用所需的表面修饰，合成并表征了基于亲和力的磁性纳米颗粒。在研究的另一部分中，将合成并表征的功能性聚合物磁性纳米颗粒用于从具有携带CD133表面标志物的癌症干细胞亚群的人骨肉瘤癌细胞系（SAOS-2）中分离癌症干细胞。通过磁珠分选法和荧光激活细胞分选法评估干细胞分离后分离的成功率和效率。结果，当His-graft-mg-p(HEMA)纳米颗粒以0.1 µg/mL的浓度用于10和10个细胞时，获得了优于商业微珠的分离效率。
