Estol P C, Poseiro J J, Schwarcz R
Centro Latinoamericano de Perinatologia y Desarrollo Humano (CLAP) PAHO/WHO, Morensea, Uruguay.
J Perinat Med. 1992;20(1):65-71. doi: 10.1515/jpme.1992.20.1.65.
Four hundred and forty seven pregnant women with ruptured membranes, were prospectively studied in order to assess the diagnostic capacity of Phosphatidylglycerol (PhG) determination in amniotic fluid recovered from vulval pads in the diagnosis of Hyaline Membrane Disease (HMD). The identification of PhG was performed using one dimensional silica gel thin layer chromatography. The sensitivity of PhG determination in the diagnosis of HMD in newborns of the total population was found to be 88.2%, with a specificity of 76.9%. In the study population, the incidence of HMD was 7.6%, the negative predictive value was 98.8% and, the positive predictive value was 24.0%. When the 265 newborns of the gestational age group of less than or equal to 34 weeks is considered, we observed an incidence of HMD of 12.1%. The diagnostic capacity of PhG in this group was shown by a sensitivity of 87.5%, a specificity of 76.4%, a positive predictive value of 33.7% and a negative predictive value of 97.8%. This method of assessment of fetal lung maturity has a diagnostic capability similar to that described by other authors, who used amniotic fluid obtained vaginally or transabdominally. The procedure described here of amniotic fluid collection is non-invasive, harmless to the mother and fetus and simple to perform. The characteristics of this method, allow serial studies of the amniotic fluid to be carried out.
对447例胎膜破裂的孕妇进行了前瞻性研究，以评估从外阴垫收集的羊水中磷脂酰甘油（PhG）测定在诊断透明膜病（HMD）中的诊断能力。使用一维硅胶薄层色谱法鉴定PhG。在总人口中，PhG测定对新生儿HMD诊断的敏感性为88.2%，特异性为76.9%。在研究人群中，HMD的发生率为7.6%，阴性预测值为98.8%，阳性预测值为24.0%。当考虑孕周小于或等于34周的265例新生儿时，我们观察到HMD的发生率为12.1%。该组中PhG的诊断能力表现为敏感性87.5%、特异性76.4%、阳性预测值33.7%和阴性预测值97.8%。这种评估胎儿肺成熟度的方法具有与其他作者所描述的相似的诊断能力，其他作者使用经阴道或经腹获得的羊水。这里描述的羊水收集程序是非侵入性的，对母亲和胎儿无害且操作简单。该方法的特点允许对羊水进行系列研究。
