Lockitch G, Wittmann B K, Mura S M, Hawkley L C
Clin Chem. 1984 Jul;30(7):1233-7.
Results of the "Amniostat-FLM" assay, a rapid semiquantitative test for phosphatidylglycerol, were compared with determinations of the lecithin/sphingomyelin (L/S) ratio, with phosphatidylglycerol measured by two-dimensional thin-layer chromatography, and with results of the "shake test" for 94 specimens of amniotic fluid. Correlation between results with the Amniostat and the other tests was excellent. All four tests are very accurate when predicting lung maturity. The predictive value of a negative test, i.e., that hyaline membrane disease would not occur, was between 92 and 100%. However, the accuracy of predicted lung immaturity is poor. For all four tests, predictions of lung immaturity were incorrect in more than 50% of the cases. Of the 49 infants born within 72 h of testing, none developed hyaline membrane disease when phosphatidylglycerol was detectable by either method or when the shake test indicated fetal lung maturity, but three infants with L/S ratio greater than 2/1 did develop the disease. The Amniostat provides a rapid screening method for detecting phosphatidylglycerol in amniotic fluid, which could well replace the thin-layer chromatographic method for measuring phosphatidylglycerol in the panel of diagnostic tests for fetal lung maturity.
将“羊水磷脂酰甘油快速半定量检测（Amniostat-FLM）”试验的结果，与卵磷脂/鞘磷脂（L/S）比值的测定结果、通过二维薄层色谱法测定的磷脂酰甘油结果以及94份羊水样本的“振荡试验”结果进行了比较。Amniostat试验结果与其他试验结果之间的相关性非常好。在预测肺成熟度时，所有这四项试验都非常准确。阴性试验（即不会发生透明膜病）的预测值在92%至100%之间。然而，预测肺未成熟的准确性较差。对于所有这四项试验，在超过50%的病例中，对肺未成熟的预测是错误的。在检测后72小时内出生的49名婴儿中，当通过任何一种方法可检测到磷脂酰甘油或振荡试验表明胎儿肺成熟时，没有婴儿发生透明膜病，但有三名L/S比值大于2/1的婴儿确实发生了该病。Amniostat提供了一种快速筛查方法，用于检测羊水中的磷脂酰甘油，在胎儿肺成熟度诊断试验组合中，它很可能取代测量磷脂酰甘油的薄层色谱法。
