Lakovnikov E A, Parfenov A F
Morfologiia. 2005;127(1):41-2.
The aim of this research was to study the cytological composition of spleen parenchyma in two and four month-old piglets and the distribution of cells among the major histological structures of the organ. Material was obtained from 20 decapitated piglets, tissue samples were processed using standard histological and histochemical methods including Perls, MacManus and Brachet staining. The data on the age aspect of quantitative composition and cell concentration in the spleen were obtained. The number of plasma cells in the mantle zone of lymphoid nodules was found to increase by 46%, while the number of eosinophilic granulocytes and macrophages in the red pulp increased by 67 and 40%, respectively. The number of megakaryocytes in the splenic cords increased by 127%. Population of T- and B-dependent zones with lymphocytes, plasma cells and macrophages was detected. Cellular composition and concentration changes in various splenic zones are indicative of a certain increase of organ reactivity in 4 month-old piglets.
本研究的目的是研究2月龄和4月龄仔猪脾脏实质的细胞学组成以及细胞在该器官主要组织结构中的分布。材料取自20只断头仔猪，组织样本采用标准组织学和组织化学方法处理，包括Perls、MacManus和Brachet染色。获得了脾脏定量组成和细胞浓度的年龄相关数据。发现淋巴小结套区浆细胞数量增加了46%，而红髓中嗜酸性粒细胞和巨噬细胞数量分别增加了67%和40%。脾索中巨核细胞数量增加了127%。检测到T细胞和B细胞依赖区存在淋巴细胞、浆细胞和巨噬细胞。不同脾区的细胞组成和浓度变化表明4月龄仔猪器官反应性有一定增加。
