Khlystova Z S, Podoprigora G I
Arkh Anat Gistol Embriol. 1978 Feb;74(2):36-42.
In germfree rats the splenic lymphopoiesis is not demonstrated by the investigation to be suppressed, and lymphoid follicules undergo age changes. Up to 15 days, periarterial zone of T-lymphocytes, responsible for cell immunity, is determined, and by the 30th day, as in control animals, peripheral zone of B-lymphocytes, responsible for hormonal immunity, is distinctly observed. In 4-month-old germfree animals, a large amount of free iron crystals is detected in the spleen, while in the organ of control animals its amount is still small. In 10-month-old germfree rats, with the appearance of reactive centers, the zone of B-lymphocytes widens in lymphoid follicules and iron crystals integrate in the red pulp. Lymphopoiesis in the spleen of the germfree rats seems to be maintained by certain local conditions which are connected with increased haemolytic function of the organ. This produces a discharge of a large amount of the products of erythrocytosis which, like autoantigens, stimulate lymphopoiesis in the spleen even when microflora is absent in the organism, while lymphopoiesis in lymph nodes in germfree animals is sharply inhibited.
在无菌大鼠中，研究表明脾脏淋巴细胞生成未受到抑制，且淋巴滤泡会经历年龄变化。在15日龄之前，可确定负责细胞免疫的T淋巴细胞的动脉周围区，到第30日时，与对照动物一样，可明显观察到负责体液免疫的B淋巴细胞的外周区。在4月龄的无菌动物中，脾脏中检测到大量游离铁晶体，而对照动物器官中的铁晶体数量仍然很少。在10月龄的无菌大鼠中，随着反应中心的出现，淋巴滤泡中B淋巴细胞区变宽，铁晶体整合到红髓中。无菌大鼠脾脏中的淋巴细胞生成似乎由某些局部条件维持，这些条件与该器官增强的溶血功能有关。这会产生大量红细胞生成产物的释放，这些产物如同自身抗原一样，即使机体中没有微生物群，也会刺激脾脏中的淋巴细胞生成，而无菌动物淋巴结中的淋巴细胞生成则受到明显抑制。
