Sengers B G, van Donkelaar C C, Oomens C W J, Baaijens F P T
Eindohoven University of Technology, Department of Biomedical Engineering, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
Biotechnol Prog. 2005 Jul-Aug;21(4):1252-61. doi: 10.1021/bp0500157.
Different culture conditions for cartilage tissue engineering were evaluated with respect to the supply of oxygen and glucose and the accumulation of lactate. A computational approach was adopted in which the culture configurations were modeled as a batch process and transport was considered within constructs seeded at high cell concentrations and of clinically relevant dimensions. To assess the extent to which mass transfer can be influenced theoretically, extreme cases were distinguished in which the culture medium surrounding the construct was assumed either completely static or well mixed and fully oxygenated. It can be concluded that severe oxygen depletion and lactate accumulation can occur within constructs for cartilage tissue engineering. However, the results also indicate that transport restrictions are not insurmountable, providing that the medium is well homogenized and oxygenated and the construct's surfaces are sufficiently exposed to the medium. The large variation in uptake rates of chondrocytes indicates that for any specific application the quantification of cellular utilization rates, depending on the cell source and culture conditions, is an essential starting point for optimizing culture protocols.
针对氧气和葡萄糖供应以及乳酸积累情况，评估了软骨组织工程的不同培养条件。采用了一种计算方法，将培养配置建模为间歇过程，并考虑在高细胞浓度接种且具有临床相关尺寸的构建体内的传输。为了从理论上评估传质受影响的程度，区分了极端情况，即假设构建体周围的培养基完全静止或充分混合且充分充氧。可以得出结论，软骨组织工程构建体内可能会出现严重的氧气消耗和乳酸积累。然而，结果也表明，只要培养基充分均匀化和充氧，且构建体表面充分暴露于培养基中，传输限制并非无法克服。软骨细胞摄取率的巨大差异表明，对于任何特定应用，根据细胞来源和培养条件对细胞利用率进行量化，是优化培养方案的重要起点。
