Zhou Xiaofeng, Jordan Richard C K, Li Yang, Huang Bau-Lin, Wong David T W
Dental Research Institute, School of Dentistry, University of California at Los Angeles, Los Angeles, CA.
Cancer Genet Cytogenet. 2005 Aug;161(1):86-9. doi: 10.1016/j.cancergencyto.2005.01.004.
Allelic imbalance is characteristic of oral squamous cell carcinoma (SCC) and contributes to the tumorigenesis of this disease. Our previous studies suggest that chromosome regions 8p and 11q22.2 approximately q22.3 are frequent sites of loss of heterozygosity (LOH) in head and neck SCC. Here, we explored the allelic imbalance pattern of these regions in 27 cases of oral epithelial dysplastic lesions. A previously reported frequent LOH (9p21) in head and neck dysplasia was also examined. Laser capture microdissection (LCM) technology was utilized to harvest homogenous cell populations from archived clinical tissues and thus greatly enhancing the sensitivity, accuracy and reliability of genetic assessment. The allelic imbalance (LOH and microsatellite instability) on 8p, 11q22.2 approximately q22.3, and 9p21 were observed at one or more loci in 66.7%, 63.0%, and 63.0% of cases, respectively. Our results demonstrate that 8p, 11q22.2 approximately q22.3, and 9p21 are frequent allelic imbalance regions in oral premalignant dysplasia and suggest the presence of tumor suppressor genes in these regions.
等位基因不平衡是口腔鳞状细胞癌（SCC）的特征，并且促成了该疾病的肿瘤发生。我们之前的研究表明，染色体区域8p以及11q22.2至大约q22.3是头颈部SCC中杂合性缺失（LOH）的常见位点。在此，我们探究了27例口腔上皮发育异常病变中这些区域的等位基因不平衡模式。还检测了先前报道的头颈部发育异常中常见的LOH（9p21）。利用激光捕获显微切割（LCM）技术从存档的临床组织中获取同质细胞群体，从而极大地提高了基因评估的敏感性、准确性和可靠性。在66.7%、63.0%和63.0%的病例中，分别在一个或多个位点观察到了8p、11q22.2至大约q22.3以及9p21上的等位基因不平衡（LOH和微卫星不稳定性）。我们的结果表明，8p、11q22.2至大约q22.3以及9p21是口腔癌前发育异常中常见的等位基因不平衡区域，并提示这些区域存在肿瘤抑制基因。
