Roz L, Wu C L, Porter S, Scully C, Speight P, Read A, Sloan P, Thakker N
Department of Oral Medicine, Eastman Dental Institute for Oral Health Care Sciences, London, United Kingdom.
Cancer Res. 1996 Mar 15;56(6):1228-31.
We have demonstrated previously a loss of constitutional heterozygosity on the short arm of chromosome 3 in approximately 50% of oral squamous cell carcinomas. In the present study, we have investigated 30 oral dysplastic lesions (DLs), presenting clinically as either erythroplakias or leukoplakias with histopathological features of either severe epithelial dysplasia or carcinoma in situ, for LOH on chromosome 3p using 15 microsatellite markers. Thirteen of the 30 LDs (approximately 43%) showed allelic imbalance at one or more loci. The pattern of loss in these lesions defined three noncontiguous regions of interstitial deletions that overlap with those defined for oral squamous cell carcinomas. These data indicate that the alteration of tumor suppressor genes on chromosome 3p is probably an early event in oral carcinogenesis. Additionally, 7 of the 30 DLs showed microsatellite instability. However, the frequency of loci showing microsatellite instability per lesion was low.
我们之前已证明，在大约50%的口腔鳞状细胞癌中，3号染色体短臂存在染色体组杂合性缺失。在本研究中，我们使用15个微卫星标记，对30个口腔发育异常病变（DLs）进行了研究，这些病变临床上表现为红斑或白斑，组织病理学特征为重度上皮发育异常或原位癌，以检测3号染色体短臂上的杂合性缺失（LOH）。30个DLs中有13个（约43%）在一个或多个位点显示出等位基因失衡。这些病变中的缺失模式定义了三个不连续的间质缺失区域，这些区域与口腔鳞状细胞癌所定义的区域重叠。这些数据表明，3号染色体短臂上肿瘤抑制基因的改变可能是口腔癌发生过程中的早期事件。此外，30个DLs中有7个显示出微卫星不稳定性。然而，每个病变中显示微卫星不稳定性的位点频率较低。
