噬菌体phiKZ头部的三维冷冻电子显微镜结构。

A three-dimensional cryo-electron microscopy structure of the bacteriophage phiKZ head.

作者信息

Fokine Andrei, Kostyuchenko Victor A, Efimov Andrey V, Kurochkina Lidia P, Sykilinda Nina N, Robben Johan, Volckaert Guido, Hoenger Andreas, Chipman Paul R, Battisti Anthony J, Rossmann Michael G, Mesyanzhinov Vadim V

机构信息

Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-2054, USA.

出版信息

J Mol Biol. 2005 Sep 9;352(1):117-24. doi: 10.1016/j.jmb.2005.07.018.

DOI:10.1016/j.jmb.2005.07.018

PMID:16081102

Abstract

The three-dimensional structure of the Pseudomonas aeruginosa bacteriophage phiKZ head has been determined by cryo-electron microscopy and image reconstruction to 18A resolution. The head has icosahedral symmetry measuring 1455 A in diameter along 5-fold axes and a unique portal vertex to which is attached an approximately 1800 A-long contractile tail. The 65 kDa major capsid protein, gp120, is organized into a surface lattice of hexamers, with T = 27 triangulation. The shape and size of the hexamers is similar to the hexameric building blocks of the bacteriophages T4, phi29, P22, and HK97. Pentameric vertices of the capsid are occupied by complexes composed of several special vertex proteins. The double-stranded genomic DNA is packaged into a highly condensed series of layers, separated by 24 A, that follow the contour of the inner wall of the capsid.

摘要

通过冷冻电子显微镜和图像重建技术，已将铜绿假单胞菌噬菌体phiKZ头部的三维结构解析至18埃分辨率。该头部具有二十面体对称性，沿五重轴直径为1455埃，且有一个独特的门户顶点，其上附着一条约1800埃长的收缩尾。65 kDa的主要衣壳蛋白gp120组成了一个六聚体表面晶格，三角剖分T = 27。六聚体的形状和大小与噬菌体T4、phi29、P22和HK97的六聚体结构单元相似。衣壳的五聚体顶点由几种特殊顶点蛋白组成的复合物占据。双链基因组DNA被包装成一系列高度浓缩的层，层间距为24埃，这些层沿着衣壳内壁的轮廓排列。