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噬菌体 ΦKZ 的衣壳结构为巨型噬菌体的组装和稳定提供了线索。

Capsid structure of bacteriophage ΦKZ provides insights into assembly and stabilization of jumbo phages.

机构信息

School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, Guangdong, China.

Shenzhen Key Laboratory of Pathogenic Microbes and Biosafety, Shenzhen Campus of Sun Yat-sen University, Shenzhen, Guangdong, China.

出版信息

Nat Commun. 2024 Aug 2;15(1):6551. doi: 10.1038/s41467-024-50811-1.

DOI:10.1038/s41467-024-50811-1
PMID:39095371
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11297242/
Abstract

Jumbo phages are a group of tailed bacteriophages with large genomes and capsids. As a prototype of jumbo phage, ΦKZ infects Pseudomonas aeruginosa, a multi-drug-resistant (MDR) opportunistic pathogen leading to acute or chronic infection in immunocompromised individuals. It holds potential to be used as an antimicrobial agent and as a model for uncovering basic phage biology. Although previous low-resolution structural studies have indicated that jumbo phages may have more complicated capsid structures than smaller phages such as HK97, the detailed structures and the assembly mechanism of their capsids remain largely unknown. Here, we report a 3.5-Å-resolution cryo-EM structure of the ΦKZ capsid. The structure unveiled ten minor capsid proteins, with some decorating the outer surface of the capsid and the others forming a complex network attached to the capsid's inner surface. This network seems to play roles in driving capsid assembly and capsid stabilization. Similar mechanisms of capsid assembly and stabilization are probably employed by many other jumbo viruses.

摘要

巨型噬菌体是一类具有大型基因组和衣壳的长尾噬菌体。作为巨型噬菌体的一个原型，ΦKZ 感染铜绿假单胞菌，这种多药耐药（MDR）机会性病原体可导致免疫功能低下个体的急性或慢性感染。它有潜力被用作一种抗菌剂，并作为揭示基本噬菌体生物学的模型。尽管之前的低分辨率结构研究表明，巨型噬菌体的衣壳结构可能比 HK97 等较小的噬菌体更为复杂，但它们的衣壳的详细结构和组装机制在很大程度上仍然未知。在这里，我们报告了 ΦKZ 衣壳的 3.5 Å 分辨率冷冻电镜结构。该结构揭示了十个次要衣壳蛋白，其中一些装饰在衣壳的外表面，另一些形成附着在衣壳内表面的复杂网络。该网络似乎在驱动衣壳组装和衣壳稳定方面发挥作用。许多其他巨型病毒可能采用类似的衣壳组装和稳定机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/2c9ae2872b29/41467_2024_50811_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/d92200417467/41467_2024_50811_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/eca2f1c90d5d/41467_2024_50811_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/9630a828729d/41467_2024_50811_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/10b3036641df/41467_2024_50811_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/4bdef5d4ed70/41467_2024_50811_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/2c9ae2872b29/41467_2024_50811_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/d92200417467/41467_2024_50811_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/eca2f1c90d5d/41467_2024_50811_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/9630a828729d/41467_2024_50811_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/10b3036641df/41467_2024_50811_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/4bdef5d4ed70/41467_2024_50811_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/601b/11297242/2c9ae2872b29/41467_2024_50811_Fig6_HTML.jpg

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