Hussien Eman M, El-Sayed Hanan Z, El-Moamly Amal A, Helmy Moshira M, Shaban Mohamed M
Department of Parasitology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
J Egypt Soc Parasitol. 2005 Aug;35(2):699-710.
Molecular typing of 20 Egyptian Trichomonas vaginalis clinical isolates was performed using the Restriction Fragment Length Polymorphism (RFLP) analysis employing a probe from the heat-inducible cytoplasmic HSP70 gene family hybridized with EcoR l-digested genomic DNA. In each of the isolates tested 5 to 6 distinct DNA fragments ranging from 2.7 Kb to 7.5 Kb in size were detected. Analysis of 13 isolates from symptomatic and 7 isolates from asymptomatic women revealed 6 distinct RFLP pattern subtypes of T. vaginalis. Eleven isolates (55%) showed the same RFLP pattern, teen of them (90.9%) were from symptomatic patients. T. vaginalis virus (TVV) was present in 7 isolates (35%). Only one isolate was considered resistant to Metronidazole. There were no relations between TVV infection or Metronidazole susceptibility and RFLP subtypes.
利用限制性片段长度多态性（RFLP）分析，使用来自热诱导细胞质HSP70基因家族的探针与经EcoR I消化的基因组DNA杂交，对20株埃及阴道毛滴虫临床分离株进行分子分型。在每个测试的分离株中，检测到5至6个大小在2.7 Kb至7.5 Kb之间的不同DNA片段。对13例有症状女性的分离株和7例无症状女性的分离株进行分析，发现了阴道毛滴虫的6种不同RFLP模式亚型。11株分离株（55%）显示相同的RFLP模式，其中10株（90.9%）来自有症状患者。7株分离株（35%）存在阴道毛滴虫病毒（TVV）。仅1株分离株被认为对甲硝唑耐药。TVV感染或甲硝唑敏感性与RFLP亚型之间无关联。
