Crucitti T, Abdellati S, Van Dyck E, Buvé A
STD/HIV Research & Intervention Unit, Institute of Tropical Medicine, Antwerp, Belgium.
Clin Microbiol Infect. 2008 Sep;14(9):844-52. doi: 10.1111/j.1469-0691.2008.02034.x.
Human trichomoniasis, caused by the protozoan Trichomonas vaginalis, is a highly prevalent sexually transmitted infection. However, little is known about the degree of strain variability of T. vaginalis. A reliable classification method for T. vaginalis strains would be a useful tool in the study of the epidemiology, pathogenesis and transmission of T. vaginalis. A PCR-restriction fragment length polymorphism typing method was designed and evaluated using T. vaginalis isolates obtained after culture of vaginal specimens collected in the Democratic Republic of Congo and in Zambia. The variation of the actin gene of T. vaginalis was determined for three ATCC reference strains and 151 T. vaginalis isolates. Eight different types were identified, on the basis of the digestion patterns of the amplified actin gene, with each of the restriction enzymes HindII, MseI and RsaI. It was determined that the ATCC reference strains 30001, 30240 and 50141 were of actin genotypes G, H and E, respectively. The actin genotype type E was more common in the Democratic Republic of Congo, whereas type G was the commonest type in Zambia. Translation of the nucleotide sequence showed up to three amino acid substitutions. We developed a reproducible, sensitive and specific typing method for T. vaginalis, and were able to distinguish at least eight T. vaginalis actin genotypes. Further studies are needed to evaluate the method using clinical specimens and to determine the utility of the typing method for the genotypic characterization of T. vaginalis.
由原生动物阴道毛滴虫引起的人类滴虫病是一种高度流行的性传播感染。然而，关于阴道毛滴虫菌株的变异程度知之甚少。一种可靠的阴道毛滴虫菌株分类方法将是研究阴道毛滴虫流行病学、发病机制和传播的有用工具。使用从刚果民主共和国和赞比亚收集的阴道标本培养后获得的阴道毛滴虫分离株，设计并评估了一种聚合酶链反应-限制性片段长度多态性分型方法。测定了三株美国典型培养物保藏中心（ATCC）参考菌株和151株阴道毛滴虫分离株的肌动蛋白基因变异情况。根据扩增的肌动蛋白基因用限制性内切酶HindII、MseI和RsaI的酶切图谱，鉴定出八种不同类型。确定ATCC参考菌株30001、30240和50141的肌动蛋白基因型分别为G、H和E。肌动蛋白基因型E在刚果民主共和国更为常见，而基因型G在赞比亚是最常见的类型。核苷酸序列翻译显示最多有三个氨基酸替换。我们开发了一种可重复、灵敏且特异的阴道毛滴虫分型方法，并且能够区分至少八种阴道毛滴虫肌动蛋白基因型。需要进一步研究以使用临床标本评估该方法，并确定该分型方法用于阴道毛滴虫基因分型的效用。
