Stiles J K, Shah P H, Xue L, Meade J C, Lushbaugh W B, Cleary J D, Finley R W
Department of Medicine, University of Mississippi Medical Center, Jackson 39216-4505, USA.
Am J Trop Med Hyg. 2000 Apr;62(4):441-5. doi: 10.4269/ajtmh.2000.62.441.
Subtyping isolates of Trichomonas vaginalis is an essential tool for understanding the epidemiology of this common sexually-transmitted disease. Restriction fragment length polymorphism (RFLP) analysis employing a probe from the heat-inducible cytoplasmic HSP70 gene family hybridized with EcoR I-digested genomic DNA was used in the molecular typing of Trichomonas isolates. Analysis of five American Type Culture Collection (ATCC) reference strains and 31 Jackson, Mississippi, isolates from six male and 21 female patients, revealed 10 distinct RFLP pattern subtypes of Trichomonas. The subtypes were temporally stable and cosmopolitan. The RFLP profiles seen in Maryland, Ohio, Massachusetts, and New York ATCC strains were identical to those of some Mississippi isolates, even though the samples were isolated 10-35 years apart. There was no correlation between metronidazole resistance and RFLP subtype with resistant isolates from eight patients distributed among six different subtypes.
阴道毛滴虫分离株的亚型分类是了解这种常见性传播疾病流行病学的重要工具。采用来自热诱导细胞质HSP70基因家族的探针与经EcoR I消化的基因组DNA杂交的限制性片段长度多态性（RFLP）分析被用于阴道毛滴虫分离株的分子分型。对五个美国典型培养物保藏中心（ATCC）参考菌株以及来自密西西比州杰克逊市的6名男性和21名女性患者的31株分离株进行分析，发现了10种不同的阴道毛滴虫RFLP模式亚型。这些亚型在时间上是稳定的且具有世界性。在马里兰州、俄亥俄州、马萨诸塞州和纽约的ATCC菌株中观察到的RFLP图谱与一些密西西比州分离株的图谱相同，尽管样本采集时间相隔10 - 35年。在分布于六种不同亚型中的八名患者的甲硝唑耐药分离株中，甲硝唑耐药性与RFLP亚型之间没有相关性。
